Adenoviral interleukin-3 gene-radiation therapy for prostate cancer in mouse model

Purpose: The radiosensitizing effect of IL-3 gene therapy was evaluated on the syngeneic mouse prostate cancer model. Methods and Materials: An adenoviral vector was used to deliver the mIL-3 alpha gene into syngeneic murine prostate (TRAMP-Cl) cancer cells growing in a subcutaneous site and the tumor response to irradiation was assessed. Results: Ad-mIL-3 gene therapy showed no tumor growth delay without radiation. However, intratumoral Ad-mIL-3 injection with radiation therapy showed marked tumor growth delay that was significantly greater than that of radiation alone. Conclusions: The combined intratumoral Ad-mIL-3 gene therapy and radiation therapy is a valuable option for further clinical evaluation. (C) 2004 Elsevier Inc.