Triptolide Induces S Phase Arrest and Apoptosis in Gallbladder Cancer Cells

被引：31

作者：

Hu, Yun-Ping ^{[1
,2
,3
]}

Tan, Zhu-Jun ^{[1
,2
,3
]}

Wu, Xiang-Song ^{[1
,2
,3
]}

Liu, Tian-Yu ^{[1
,2
,3
]}

Jiang, Lin ^{[1
,2
,3
]}

Bao, Run-Fa ^{[1
,2
,3
]}

Shu, Yi-Jun ^{[1
,2
,3
]}

Li, Mao-Lan ^{[1
,2
,3
]}

Weng, Hao ^{[1
,2
,3
]}

Ding, Qian ^{[1
,2
,3
]}

Tao, Feng ^{[4
]}

Liu, Ying-Bin ^{[1
,2
,3
]}

机构：

[1] Shanghai Jiao Tong Univ, Xinhua Hosp, Inst Biliary Tract Dis, Sch Med, Shanghai 200092, Peoples R China

[2] Shanghai Jiao Tong Univ, Xinhua Hosp, Lab Gen Surg, Sch Med, Shanghai 200092, Peoples R China

[3] Shanghai Jiao Tong Univ, Sch Med, Dept Gen Surg, Shanghai 200092, Peoples R China

[4] Zhejiang Univ, Shaoxing Hosp, Shaoxing Peoples Hosp, Shaoxing 312000, Peoples R China

来源：

MOLECULES | 2014年 / 19卷 / 02期

基金：

中国国家自然科学基金;

关键词：

gallbladder cancer cells; triptolide; proliferation; cell cycle; apoptosis; KAPPA-B ACTIVATION; CARCINOMA METASTASIS; GALL-BLADDER; PROTEIN; DEATH; INHIBITION; GROWTH; HOOK;

D O I：

10.3390/molecules19022612

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Gallbladder carcinoma is the most common malignancy of the biliary tract, with a very low 5-year survival rate and extremely poor prognosis. Thus, new effective treatments and drugs are urgently needed for the treatment of this malignancy. In this study, for the first time we investigated the effects of triptolide on gallbladder cancer cells and identified the mechanisms underlying its potential anticancer effects. The MTT assay showed that triptolide decreased cell viability in a dose-and time-dependent manner. The results of the colony formation assay indicated that triptolide strongly suppressed colony formation ability in GBC-SD and SGC-996 cells. Flow cytometric analysis revealed that triptolide induced S phase arrest in gallbladder cancer cells. In addition, triptolide induced apoptosis, as shown by the results of annexin V/propidium iodide double-staining and Hoechst 33342 staining. Furthermore, triptolide decreased mitochondrial membrane potential (Delta Psi m) in a dose-dependent manner. Finally, western blot analysis of triptolide-treated cells revealed the activation of caspase-3, caspase-9, PARP, and Bcl-2; this result demonstrated that triptolide induced apoptosis in gallbladder cancer cells by regulating apoptosis-related protein expression, and suggests that triptolide may be a promising drug to treat gallbladder carcinoma.

引用

页码：2612 / 2628

页数：17

共 36 条

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