Cells transformed by a v-Myb-estrogen receptor fusion differentiate into multinucleated giant cells

被引:13
作者
Engelke, U [1 ]
Wang, DM [1 ]
Lipsick, JS [1 ]
机构
[1] STANFORD UNIV,SCH MED,DEPT PATHOL,STANFORD,CA 94305
关键词
D O I
10.1128/JVI.71.5.3760-3766.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In order to make conditional alleles of the v-myb oncogene, we constructed and tested avian retroviruses which produce a number of different fusion proteins between v-Myb and the human estrogen receptor (ER), We found that the portion of the ER used in making these fusions profoundly affected their transcriptional activation, However, all the fusions tested were only weakly transforming in embryonic yolk sac assays and there was no direct correlation between the level of transcriptional activation and strength of oncogenic transformation, Nevertheless, transformation by a v-Myb-ER fusion was estrogen dependent, and upon withdrawal of the hormone, monocytic-lineage cells differentiated into multinucleated giant cells, Surprisingly, the withdrawal of estrogen caused a dramatic increase in the stability of the fusion protein, although it remained unable to promote cell growth or block differentiation.
引用
收藏
页码:3760 / 3766
页数:7
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