Specificity and versatility in erythromycin biosynthesis

被引：9

作者：

Pieper, R

Kao, C

Khosla, C

Luo, GL

Cane, DE

机构：

[1] STANFORD UNIV,DEPT CHEM ENGN,STANFORD,CA 94305

[2] BROWN UNIV,DEPT CHEM,PROVIDENCE,RI 02912

来源：

CHEMICAL SOCIETY REVIEWS | 1996年 / 25卷 / 05期

关键词：

D O I：

10.1039/cs9962500297

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

6-Deoxyerythronolide B synthase is a modular polyketide synthase consisting of three large, multienzyme proteins with at least 28 distinct active sites that together catalyse the formation of 6-deoxyerythronolide B, the parent macrolide of the medicinally important erythromycin family of broad spectrum antibiotics. The combined use of molecular genetic and enzyme mechanistic tools has provided exciting new insights into the organization and function of these fascinating catalysts and allowed the rational generation of novel 'unnatural' natural products.

引用

页码：297 / &

页数：7

共 32 条

[1] APARICIO JF, 1994, J BIOL CHEM, V269, P8524
[2] A MUTANT GENERATED BY EXPRESSION OF AN ENGINEERED DEBS1 PROTEIN FROM THE ERYTHROMYCIN-PRODUCING POLYKETIDE SYNTHASE (PKS) IN STREPTOMYCES-COELICOLOR PRODUCES THE TRIKETIDE AS A LACTONE, BUT THE MAJOR PRODUCT IS THE NOR-ANALOG DERIVED FROM ACETATE AS STARTER ACID
BROWN, MJB
CORTES, J
CUTTER, AL
LEADLAY, PF
STAUNTON, J
[J]. JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1995, (15) : 1517 - 1518
[3] IDENTIFICATION OF DEBS-1, DEBS-2 AND DEBS-3, THE MULTIENZYME POLYPEPTIDES OF THE ERYTHROMYCIN-PRODUCING POLYKETIDE SYNTHASE FROM SACCHAROPOLYSPORA-ERYTHRAEA
CAFFREY, P
BEVITT, DJ
STAUNTON, J
LEADLAY, PF
[J]. FEBS LETTERS, 1992, 304 (2-3) : 225 - 228
[4] ERYTHROMYCIN BIOSYNTHESIS - HIGHLY EFFICIENT INCORPORATION OF POLYKETIDE CHAIN ELONGATION INTERMEDIATES INTO 6-DEOXYERYTHRONOLIDE-B IN AN ENGINEERED STREPTOMYCES HOST
CANE, DE
LUO, GG
KHOSLA, C
KAO, CM
KATZ, L
[J]. JOURNAL OF ANTIBIOTICS, 1995, 48 (07) : 647 - 651
[5] MACROLIDE BIOSYNTHESIS .4. INTACT INCORPORATION OF A CHAIN-ELONGATION INTERMEDIATE INTO ERYTHROMYCIN
CANE, DE
YANG, CC
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1987, 109 (04) : 1255 - 1257
[6] MACROLIDE BIOSYNTHESIS .3. STEREOCHEMISTRY OF THE CHAIN-ELONGATION STEPS OF ERYTHROMYCIN BIOSYNTHESIS
CANE, DE
LIANG, TC
TAYLOR, PB
CHANG, C
YANG, CC
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (16) : 4957 - 4964
[7] MACROLIDE BIOSYNTHESIS .2. ORIGIN OF THE CARBON SKELETON AND OXYGEN-ATOMS OF THE ERYTHROMYCINS
CANE, DE
HASLER, H
TAYLOR, PB
LIANG, TC
[J]. TETRAHEDRON, 1983, 39 (21) : 3449 - 3455
[8] UNIFIED STEREOCHEMICAL MODEL OF POLYETHER ANTIBIOTIC-STRUCTURE AND BIOGENESIS
CANE, DE
CELMER, WD
WESTLEY, JW
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1983, 105 (11) : 3594 - 3600
[9] MACROLIDE STEREOCHEMISTRY .3. A CONFIGURATIONAL MODEL FOR MACROLIDE ANTIBIOTICS
CELMER, WD
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1965, 87 (08) : 1801 - &
[10] AN UNUSUALLY LARGE MULTIFUNCTIONAL POLYPEPTIDE IN THE ERYTHROMYCIN-PRODUCING POLYKETIDE SYNTHASE OF SACCHAROPOLYSPORA-ERYTHRAEA
CORTES, J
HAYDOCK, SF
ROBERTS, GA
BEVITT, DJ
LEADLAY, PF
[J]. NATURE, 1990, 348 (6297) : 176 - 178

← 1 2 3 4 →