Comparison of the double-disk, combined disk, and Etest methods for detecting metallo-β-lactamases in gram-negative bacilli

被引:69
作者
Yan, JJ [1 ]
Wu, JJ
Tsai, SH
Chuang, CL
机构
[1] Natl Cheng Kung Univ Hosp, Dept Pathol, Tainan 70428, Taiwan
[2] Natl Cheng Kung Univ, Coll Med, Dept Med Technol, Tainan 70101, Taiwan
关键词
D O I
10.1016/j.diagmicrobio.2004.01.002
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Three phenotypic methods for detecting metallo-beta-lactamases (MBLs) in gram-negative bacilli were tested on 60 MBL producers and 155 MBL nonproducers. The 2-mercaptopropionic acid double-disk potentiation method using ceftazidime and cefepime with and without clavulanate was found to be most sensitive (100%). The combined disk method depends on comparing the zones given by disks containing a beta-lactam with and without ethylenediaminetetraacetic acid. Various drug combinations were tested, and the best results were obtained with imipenem for Pseudomonos species and Acinetobacter baumannii, ceftazidime-clavulanate for Klebsiella pneumoniae, and cefepime-clavulanate for Enterobocter cloacae and Citrobacter freundii. The overall sensitivity of the combined disk method was 86.7%. The Etest (AB BIODISK, Solna, Sweden) method using imipenem- ethylenediaminetetraacetic acid strips detected imipenem-resistant MBL-producing isolates only, resulting in a sensitivity of 36.7%. Our data suggest that the double-disk and combined disk tests are acceptable methods for NIBL detection in both imipenem-resistant and imipenem-susceptible isolates. (C) 2004 Elsevier Inc. All rights reserved.
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页码:5 / 11
页数:7
相关论文
共 27 条
  • [1] Arakawa Y, 2000, J CLIN MICROBIOL, V38, P40
  • [2] A FUNCTIONAL CLASSIFICATION SCHEME FOR BETA-LACTAMASES AND ITS CORRELATION WITH MOLECULAR-STRUCTURE
    BUSH, K
    JACOBY, GA
    MEDEIROS, AA
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (06) : 1211 - 1233
  • [3] IMP-4, a novel metallo-β-lactamase from nosocomial Acinetobacter spp. collected in Hong Kong between 1994 and 1998
    Chu, YW
    Afzal-Shah, M
    Houang, ETS
    Palepou, MFI
    Lyon, DJ
    Woodford, N
    Livermore, DM
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (03) : 710 - 714
  • [4] Hospital outbreak of carbapenem-resistant Pseudomonas aeruginosa producing VIM-1, a novel transferable metallo-β-lactamase
    Cornaglia, G
    Mazzariol, A
    Lauretti, L
    Rossolini, GM
    Fontana, R
    [J]. CLINICAL INFECTIOUS DISEASES, 2000, 31 (05) : 1119 - 1125
  • [5] Nosocomial outbreak of carbapenem-resistant Pseudomonas aeruginosa with a new blaIMP allele, blaIMP-7
    Gibb, AP
    Tribuddharat, C
    Moore, RA
    Louie, TJ
    Krulicki, W
    Livermore, DM
    Palepou, MFI
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (01) : 255 - 258
  • [6] Occurrence of a new metallo-β-lactamase IMP-4 carried on a conjugative plasmid in Citrobacter youngae from the People's Republic of China
    Hawkey, PM
    Xiong, JH
    Ye, HF
    Li, HY
    M'Zali, FH
    [J]. FEMS MICROBIOLOGY LETTERS, 2001, 194 (01) : 53 - 57
  • [7] Rapid detection and evaluation of clinical characteristics of emerging multiple-drug-resistant gram-negative rods carrying the metallo-β-lactamase gene blaIMP
    Hirakata, Y
    Izumikawa, K
    Yamaguchi, T
    Takemura, H
    Tanaka, H
    Yoshida, R
    Matsuda, J
    Nakano, M
    Tomono, K
    Maesaki, S
    Kaku, M
    Yamada, Y
    Kamihira, S
    Kohno, S
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (08) : 2006 - 2011
  • [8] STATISTICAL-METHODS IN MICROBIOLOGY
    ILSTRUP, DM
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 1990, 3 (03) : 219 - 226
  • [9] Cloning and characterization of blaVIM, a new integron-borne metallo-β-lactamase gene from a Pseudomonas aeruginosa clinical isolate
    Lauretti, L
    Riccio, ML
    Mazzariol, A
    Cornaglia, G
    Amicosante, G
    Fontana, R
    Rossolini, GM
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (07) : 1584 - 1590
  • [10] blaVIM-2 Cassette-containing novel integrons in metallo-β-lactamase-producing Pseudomonas aeruginosa and Pseudomonas putida isolates disseminated in a Korean hospital
    Lee, K
    Lim, JB
    Yum, JH
    Yong, DG
    Chong, YS
    Kim, JM
    Livermore, DM
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (04) : 1053 - 1058