Three phenotypic methods for detecting metallo-beta-lactamases (MBLs) in gram-negative bacilli were tested on 60 MBL producers and 155 MBL nonproducers. The 2-mercaptopropionic acid double-disk potentiation method using ceftazidime and cefepime with and without clavulanate was found to be most sensitive (100%). The combined disk method depends on comparing the zones given by disks containing a beta-lactam with and without ethylenediaminetetraacetic acid. Various drug combinations were tested, and the best results were obtained with imipenem for Pseudomonos species and Acinetobacter baumannii, ceftazidime-clavulanate for Klebsiella pneumoniae, and cefepime-clavulanate for Enterobocter cloacae and Citrobacter freundii. The overall sensitivity of the combined disk method was 86.7%. The Etest (AB BIODISK, Solna, Sweden) method using imipenem- ethylenediaminetetraacetic acid strips detected imipenem-resistant MBL-producing isolates only, resulting in a sensitivity of 36.7%. Our data suggest that the double-disk and combined disk tests are acceptable methods for NIBL detection in both imipenem-resistant and imipenem-susceptible isolates. (C) 2004 Elsevier Inc. All rights reserved.
机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Microbiol, Shatin, Hong Kong, Peoples R China
Chu, YW
Afzal-Shah, M
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Afzal-Shah, M
Houang, ETS
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Chinese Univ Hong Kong, Prince Wales Hosp, Dept Microbiol, Shatin, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Prince Wales Hosp, Dept Microbiol, Shatin, Hong Kong, Peoples R China
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Palepou, MFI
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Palepou, MFI
Lyon, DJ
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Lyon, DJ
Woodford, N
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Woodford, N
Livermore, DM
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机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Microbiol, Shatin, Hong Kong, Peoples R China
机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Microbiol, Shatin, Hong Kong, Peoples R China
Chu, YW
Afzal-Shah, M
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机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Microbiol, Shatin, Hong Kong, Peoples R China
Afzal-Shah, M
Houang, ETS
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Chinese Univ Hong Kong, Prince Wales Hosp, Dept Microbiol, Shatin, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Prince Wales Hosp, Dept Microbiol, Shatin, Hong Kong, Peoples R China
Houang, ETS
Palepou, MFI
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Palepou, MFI
Lyon, DJ
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机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Microbiol, Shatin, Hong Kong, Peoples R China
Lyon, DJ
Woodford, N
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机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Microbiol, Shatin, Hong Kong, Peoples R China
Woodford, N
Livermore, DM
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机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Microbiol, Shatin, Hong Kong, Peoples R China