Expression of adenylyl cyclase mRNAs in the adult, in developing, and in the Brattleboro rat kidney

被引：32

作者：

Shen, TS ^{[1
]}

Suzuki, Y ^{[1
]}

Poyard, M ^{[1
]}

Miyamoto, N ^{[1
]}

Defer, N ^{[1
]}

Hanoune, J ^{[1
]}

机构：

[1] HOP HENRI MONDOR, INSERM, U99, F-94010 CRETEIL, FRANCE

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1997年 / 273卷 / 01期

关键词：

adenylyl cyclase activity; Northern blot; in situ hybridization; G proteins; vasopressin V-2 receptor;

D O I：

10.1152/ajpcell.1997.273.1.C323

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The activity and expression of adenylyl cyclases (AC) were examined in the adult rat renal cortex and medulla. Northern blot analysis and in situ hybridization demonstrated that AC-6 was the predominant isoform in the adult rat kidney, whereas AC-4, -5, and -9 had a lower expression. AC-4 expression was higher in the cortex, and AC-5 and AC-6 were higher in the medulla. AC-9 expression was at the same level in both regions. AC activity was high in the fetus and declined in the adult. At all stages, AC activity was sensitive to parathyroid hormone, whereas no stimulation by vasopressin and isoproterenol was found in the fetus and the neonate. AC-5 and AC-6 mRNAs increased at day 1 and then markedly decreased, paralleling the decline in AC activity. The mRNA of AC-4 did not change and that of AC-9 increased markedly until adult. In the homozygous Brattleboro rat kidney, the expression of all these isoforms was decreased.

引用

页码：C323 / C330

页数：8

共 29 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] CATECHOLAMINE SENSITIVE ADENYLATE CYCLASE ACTIVITY IN DIFFERENT SEGMENTS OF RABBIT NEPHRON [J].