Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product

被引：89

作者：

Khaskheli, Shahzor Gul ^{[1
,2
]}

Zheng, Wen ^{[1
]}

Sheikh, Saghir Ahmed ^{[2
]}

Khaskheli, Ashfaque Ahmed ^{[1
]}

Liu, Ying ^{[1
]}

Soomro, Aijaz Hussain ^{[2
]}

Feng, Xi ^{[3
]}

Sauer, Mary Ball ^{[3
]}

Wang, Yan Feng ^{[4
]}

Huang, Wen ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China

[2] Sindh Agr Univ, Inst Food Sci & Technol, Tandojam 71000, Pakistan

[3] Iowa State Univ, Dept Anim Sci, Ames, IA 50010 USA

[4] Heilongjiang Acad Agr Sci, Mudanjiang Subacad, Mudanjiang 157041, Heilongjiang, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 81卷

关键词：

Auricularia auricula; Polysaccharide; Monosaccharide composition; Antioxidant activity; Hot water extraction; WATER-SOLUBLE POLYSACCHARIDES; ASSISTED EXTRACTION; LYCIUM-BARBARUM; IN-VITRO; CAPACITY; EXPRESSION; MUSHROOMS;

D O I：

10.1016/j.ijbiomac.2015.08.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The study planned to determine proximate composition, antioxidant activity and chemical characterization in the fruit bodies of Auricularia auricula and pickled product. Two polysaccharide fractions (AAPF, AAPP) were purified from the fruiting body of A. auricula by ethanol precipitation, deproteination and macroporous resins and were subsequently lyophilized. The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0. AAPF and AAPP were characterized by Fourier Transform Infrared spectroscopy (FTIR), which indicated peaks at approximately 3500 cm(-1) and 522.28 cm(-1) in the carbohydrate region and composed of beta-glycoside linkages at the 1200-1000 cm(-1) peaks. However, the antioxidant activity of AAPF demonstrated a significant result in scavenging free radicals except for DDPH. The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：387 / 395

页数：9

共 46 条

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Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies [J].