Structural characterization of lignin during Pinus taeda wood treatment with Ceripotiopsis subvermispora

被引:77
作者
Guerra, A
Mendonça, R
Ferraz, A
Lu, FC
Ralph, J
机构
[1] Fac Engn Quim Lorena, Dept Biotechnol, BR-12600000 Sao Paulo, Brazil
[2] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA
关键词
D O I
10.1128/AEM.70.7.4073-4078.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Pinus taeda wood chips were biotreated with Ceriporiopsis subvermispora under solid-state fermentation for periods varying from 15 to 90 days. Milled wood lignins extracted from sound and biotreated wood samples were characterized by wet-chemical and spectroscopic techniques. Treatment of the lignins by derivatization followed by reductive cleavage (DFRC) made it possible to detect DFRC monomers and dimers that are diagnostic of the occurrence of arylglycerol-beta-o-aryl and beta-beta, beta-5, beta-1, and 4-O-5 units in the lignin structure. Quantification of these DFRC products indicated that beta-O-aryl cleavage was a significant route for lignin biodegradation but that beta-beta, beta-5, beta-1, and 4-O-5 linkages were more resistant to the biological attack. The amount of aromatic hydroxyls did not increase with the split of beta-O-4 linkages, suggesting that the beta-O-4 cleavage products remain as quinone-type structures as detected by UV and visible spectroscopy. Nuclear magnetic resonance techniques also indicated the formation of new substructures containing nonoxygenated, saturated aliphatic carbons (CH2 and CH3) in the side chains of lignins extracted from biotreated wood samples.
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页码:4073 / 4078
页数:6
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