Depletion of a fatty acid-binding protein impairs neurite outgrowth in PC12 cells

被引:29
作者
Allen, GW
Liu, JW
De León, M
机构
[1] Loma Linda Univ, Sch Med, Dept Physiol & Pharmacol, Loma Linda, CA 92350 USA
[2] Loma Linda Univ, Ctr Mol Biol & Gene Therapy, Sch Med, Loma Linda, CA 92350 USA
来源
MOLECULAR BRAIN RESEARCH | 2000年 / 76卷 / 02期
基金
美国国家科学基金会;
关键词
neurite outgrowth; nerve growth factor; fatty acid; PC12 cell differentiation;
D O I
10.1016/S0169-328X(00)00014-0
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Epithelial fatty acid-binding protein (E-FABP) is up-regulated in rat dorsal root ganglia after sciatic nerve crush and in differentiating neurons during development. The present study investigates the role of E-FABP during nerve growth factor (NGF)-mediated neurite outgrowth in PC12 cells. Undifferentiated PC12 cells express low levels of E-FABP, while NGF triggers a 6- and 8-fold induction of E-FABP mRNA and protein, respectively. Up-regulation of E-FABP mRNA occurs as early as 24 h after NGF treatment and remains highly expressed over the course of several days, corresponding to NGF-mediated neurite outgrowth. Withdrawal of NGF leads to down-regulation of E-FABP mRNA and retraction of neurites. Immunofluorescence microscopy reveals E-FABP immunoreactivity in the perinuclear cytoplasm, neurites and growth cones of NGF-differentiated cells. To examine the role of E-FABP during neurite outgrowth, PC12 cells were transfected with a constitutive antisense E-FABP vector to create the E-FABP-deficient line PC12-AS. By morphometric analysis, PC12-AS cells treated for 2, 4, and 7 days with NGF exhibited significantly decreased neurite expression relative to control (mock-transfected) cells. Taken together, these data indicate that E-FABP is important in normal NGF-mediated neurite outgrowth in PC12 cells, a finding that is consistent with a potential role in axonal development and regeneration. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:315 / 324
页数:10
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