Fragmentation of DNA in morphologically normal human spermatozoa

被引：99

作者：

Avendano, Conrado ^{[1
]}

Franchi, Anahi ^{[1
]}

Taylor, Steven ^{[1
]}

Morshedi, Mahmood ^{[1
]}

Bocca, Silvina ^{[1
]}

Oehninger, Sergio ^{[1
]}

机构：

[1] Eastern Virginia Med Sch, Jones Inst Reprod Med, Dept Obstet & Gynecol, Norfolk, VA 23507 USA

来源：

FERTILITY AND STERILITY | 2009年 / 91卷 / 04期

关键词：

DNA fragmentation; normal morphology; sperm selection; teratozoospermia; INTRACYTOPLASMIC SPERM INJECTION; INVITRO FERTILIZATION; NUCLEAR VACUOLES; PREDICTIVE VALUE; STRICT CRITERIA; DAMAGE; ICSI; EMBRYO; SEMEN; TERATOZOOSPERMIA;

D O I：

10.1016/j.fertnstert.2008.01.015

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To evaluate DNA fragmentation in spermatozoa with normal morphological appearance. Design: Prospective study. Setting: Academic tertiary center. Patient(s): Fertile, subfertile, and infertile men were studied. Intervention(s): Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-fluorescein nick-end labeling assay and morphology assessment by phase contrast in the swim-up fractions. Main Outcome Measure(s): Simultaneous assessment of the percentage of normally shaped sperm and DNA fragmentation. Result(s): No DNA fragmentation was found in spermatozoa with normal morphology in any of the samples from the fertile group. In only one sample from the subfertile group did we observed normally shaped sperm cells exhibiting DNA fragmentation. However, in all the samples from the infertile group, we observed normal spermatozoa with DNA fragmentation. Spermatozoa from this late group exhibited a high proportion of DNA damage. Conclusion(s): In infertile men with moderate and severe teratozoospermia, the spermatozoa with apparently normal morphology present in the motile fractions after swim-up may have DNA fragmentation. (Fertil Steril (R) 2009;91:1077-84. (C) 2009 by American Society for Reproductive Medicine.)

引用

页码：1077 / 1084

页数：8

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