Microbial community analysis with a high PHA storage capacity

被引:28
作者
Serafim, L. S. [1 ]
Lemos, P. C.
Rossetti, S.
Levantesi, C.
Tandoi, V.
Reis, M. A. M.
机构
[1] Univ Nova Lisboa, FCT, REQUIMTE, COFB,Dept Quim, P-2829516 Caparica, Portugal
[2] Univ Nova Lisboa, ITOB, P-2870156 Oeiras, Portugal
[3] CNR, Water Res Inst, I-00198 Rome, Italy
关键词
acetate; aerobic dynamic substrate feeding; FISH; polyhydroxyalkanoates; propionate; SBR;
D O I
10.2166/wst.2006.386
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Activated sludge was submitted to aerobic dynamic substrate feeding for the production of biodegradable plastics. Two sequencing batch reactors were operated with acetate or propionate as sole carbon substrates. With acetate a homopolymer of polyhydroxybutyrate (PHB) was obtained and with propionate a copolymer of hydroxybutyrate and hydroxyvalerate P(HB/HV) was produced. Three main morphotypes were identified in both sludges: two belong to the Alphaproteobacteria class and the third to the Betaproteobacteria class. Bacilli belonging to Betaproteobacteria were shown by FISH analysis, applied in combination with Nile Blue post-staining, to be the main responsible for PHAs storage. The latter were affiliated to Azoarcus genus within Betaproteobacteria.
引用
收藏
页码:183 / 188
页数:6
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