Kinetics of proton uptake and dye binding by photoactive yellow protein in wild type and in the E46Q and E46A mutants

We studied the kinetics of proton uptake and release by photoactive yellow protein (PYP) from Ectothiorhodospira halophila in wild type and the E46Q and E46A mutants by transient absorption spectroscopy with the pH-indicator dyes bromocresol purple or cresol red in unbuffered solution. In parallel, we investigated the kinetics of chromophore protonation as monitored by the rise and decay of the blueshifted state I-2 (lambda(max) = 355 nm). For wild type the proton uptake kinetics is synchronized with the fast phase of I-2 formation (tau = 500 mus at pH 6.2). The transient absorption signal from the dye also contains a slower component which is not due to dye deprotonation but is caused by dye binding to a hydrophobic patch that is transiently exposed in the structurally changed and partially unfolded I-2, intermediate. This conclusion is based on the wavelength, pH, and concentration dependence of the dye signal and on dye measurements in the presence of buffer. SVD analysis, moreover, indicates the presence of two components in the dye signal: protonation and dye binding. The dye binding has a rise time of about 4 ms and is coupled kinetically with a transition between two I-2 intermediates. In the mutant E46Q, which lacks the putative internal proton donor E46, the formation Of I-2 is accelerated, but the proton uptake kinetics remains kinetically coupled to the fast phase Of I-2 formation (tau = 100 mus at pH 6.3). For this mutant the protein conformational change, as monitored by the dye binding, occurs with about the same time constant as in wild type but with reduced amplitude. In the alkaline form of the mutant E46A the formation of the L-like intermediate is even faster as is the proton uptake (tau = 20 mus at pH 8.3). No dye binding occurred in E46A, suggesting the absence of a conformational change. In all of the systems proton release is synchronized with the decay of I-2. Our results support mechanisms in which the chromophore of PYP is protonated directly from the external medium rather than by the internal donor E46.