Application of Laser Microdissection and Reverse-Phase Protein Microarrays to the Molecular Profiling of Cancer Signal Pathway Networks in the Tissue Microenvironment

被引：20

作者：

Espina, Virginia ^{[1
]}

Wulfkuhle, Julia ^{[1
]}

Liotta, Lance A. ^{[1
]}

机构：

[1] George Mason Univ, Dept Mol & Microbiol, Ctr Appl Prote & Mol Med, Manassas, VA 20110 USA

来源：

CLINICS IN LABORATORY MEDICINE | 2009年 / 29卷 / 01期

关键词：

Cancer; Laser capture microdissection; Microenvironment; Molecular profiling; Proteomics; Reverse phase protein microarray; CELL LUNG-CANCER; CAPTURE MICRODISSECTION; BREAST-CANCER; COMBINATION THERAPY; PROTEOMIC ANALYSIS; END-POINTS; RECEPTOR; GEFITINIB; INHIBITORS; MUTATIONS;

D O I：

10.1016/j.cll.2009.03.001

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Laser-capture microdissection (LCM) is used to procure specific tissue cell subpopulations under direct microscopic visualization of a standard-stained frozen or fixed tissue section on a glass microscope slide. Protein microarrays can measure hundreds of analytes in a small input sample. A particular type of protein microarray, the reverse-phase array (RPA), is sensitive enough to accurately measure the small concentration of activated signal pathway molecules in microdissected tissue samples. This article explains how these two technologies, LCM and RPA, can be combined to yield molecular pathway data for the individualized therapy of the future.

引用

页码：1 / +

页数：14

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