Functionalization of Si/SiO2 substrates with homooligonucleotides for a DNA biosensor

被引：44

作者：

Cloarec, JP

Martin, JR

Polychronakos, C

Lawrence, I

Lawrence, MF

Souteyrand, E

机构：

[1] Ecole Cent Lyon, PCI, IFOS, F-69131 Ecully, France

[2] Concordia Univ, Dept Chem & Biochem, Montreal, PQ H3G 1M8, Canada

[3] Montreal Childrens Hosp, Montreal, PQ H3H 1P3, Canada

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1999年 / 58卷 / 1-3期

关键词：

Si/SiO2; substrates; homooligonucleotides; DNA biosensors;

D O I：

10.1016/S0925-4005(99)00102-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Si/SiO2 substrates bearing single stranded homooligonucleotidic sequences are implemented, in order to use as DNA biosensors based on field effect measurement. The oligonucleotides are immobilized on the substrates by two different methods, with either an aminosilane or a glycidoxysilane. We present investigations and characterizations of both methods of immobilization, using radiolabelling and electrochemical impedance measurements on electrolyte/dielectric/semiconductor heterostructures. In our conditions of reaction, the protocol using glycidoxysilane allows a denser and more robust immobilization of oligonucleotides than with the aminosilane. (C) 1999 Elsevier Science S.A. All rights reserved.

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页码：394 / 398

页数：5

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