Role of His243 in the phosphatase activity of EnvZ in Escherichia coli

被引：30

作者：

Skarphol, K ^{[1
]}

Waukau, J ^{[1
]}

Forst, SA ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT BIOL SCI,MILWAUKEE,WI 53201

来源：

JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 04期

关键词：

D O I：

10.1128/jb.179.4.1413-1416.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

EnvZ undergoes autophosphorylation at His243 and subsequently transfers the phosphate group to OmpR. EnvZ also possesses an OmpR-phosphate phosphatase activity. We examined the role of His243 in the phosphatase function by replacing His with either Val, Tyr, Ser, Asp, or Asn. EnvZH243V and EnvZH243Y were both shown to possess phosphatase activity in vitro. In addition, the mutant proteins were able to reduce the high level of OmpR-phosphate present in the envZ473 strain. These results indicate that His243 of EnvZ is not essential for stimulating the dephosphorylation of OmpR-phosphate.

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页码：1413 / 1416

页数：4

相关论文

共 23 条

[1]

AIBA H, 1989, J BIOL CHEM, V264, P14090

[2] MUTATIONAL ANALYSIS OF THE BACTERIAL SIGNAL-TRANSDUCING PROTEIN-KINASE PHOSPHATASE NITROGEN REGULATOR-II (NR(II) OR NTR(B)) [J].

ATKINSON, MR ;

NINFA, AJ .

JOURNAL OF BACTERIOLOGY, 1993, 175 (21) :7016-7023

[3] THE NARX AND NARQ SENSOR-TRANSMITTER PROTEINS OF ESCHERICHIA-COLI EACH REQUIRE 2 CONSERVED HISTIDINES FOR NITRATE-DEPENDENT SIGNAL-TRANSDUCTION TO NARL [J].

CAVICCHIOLI, R ;

SCHRODER, I ;

CONSTANTI, M ;

GUNSALUS, RP .

JOURNAL OF BACTERIOLOGY, 1995, 177 (09) :2416-2424

[4] IDENTIFICATION OF A PHOSPHORYLATION SITE AND FUNCTIONAL-ANALYSIS OF CONSERVED ASPARTIC-ACID RESIDUES OF OMPR, A TRANSCRIPTIONAL ACTIVATOR FOR OMPF AND OMPC IN ESCHERICHIA-COLI [J].

DELGADO, J ;

FORST, S ;

HARLOCKER, S ;

INOUYE, M .

MOLECULAR MICROBIOLOGY, 1993, 10 (05) :1037-1047

[5] Reverse phosphotransfer from OmpR to EnvZ in a kinase(-)/phosphatase(+) mutant of EnvZ (EnvZ center dot N347D), a bifunctional signal transducer of Escherichia coli [J].

Dutta, R ;

Inouye, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (03) :1424-1429

[6] INVIVO PHOSPHORYLATION OF OMPR, THE TRANSCRIPTION ACTIVATOR OF THE OMPF AND OMPC GENES IN ESCHERICHIA-COLI [J].

FORST, S ;

DELGADO, J ;

RAMPERSAUD, A ;

INOUYE, M .

JOURNAL OF BACTERIOLOGY, 1990, 172 (06) :3473-3477

[7] PHOSPHORYLATION OF OMPR BY THE OSMOSENSOR ENVZ MODULATES EXPRESSION OF THE OMPF AND OMPC GENES IN ESCHERICHIA-COLI [J].

FORST, S ;

DELGADO, J ;

INOUYE, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6052-6056

[8] SIGNAL-TRANSDUCTION BY THE ENVZ-OMPR PHOSPHOTRANSFER SYSTEM IN BACTERIA [J].

FORST, SA ;

ROBERTS, DL .

RESEARCH IN MICROBIOLOGY, 1994, 145 (5-6) :363-373

[9] OSMOREGULATION OF THE FATTY-ACID RECEPTOR GENE FADL IN ESCHERICHIA-COLI [J].

HIGASHITANI, A ;

NISHIMURA, Y ;

HARA, H ;

AIBA, H ;

MIZUNO, T ;

HORIUCHI, K .

MOLECULAR & GENERAL GENETICS, 1993, 240 (03) :339-347

[10]

IGO M M, 1990, New Biologist, V2, P5