Cyclin D3 interacts with human activating transcription factor 5 and potentiates its transcription activity

被引：28

作者：

Liu, WJ

Sun, MY

Jiang, JH

Shen, XY

Sun, Q

Liu, WC

Shen, HL

Gu, JX ^{[1
]}

机构：

[1] Fudan Univ, Shanghai Med Coll, State Key Lab Genet Engn, Shanghai 200032, Peoples R China

[2] Fudan Univ, Shanghai Med Coll, Ctr Gene Res, Shanghai 200032, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 321卷 / 04期

基金：

中国国家自然科学基金;

关键词：

cyclin D3; hATF5; interact; transcriptional regulation;

D O I：

10.1016/j.bbrc.2004.07.053

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Cyclin D3 protein is a member of the D-type cyclins. Besides serving as cell cycle regulators, D-type cyclins have been reported to be able to interact with several transcription factors and modulate their transcriptional activations. Here we report that human activating transcription factor 5 (hATF5) is a new interacting partner of Cyclin D3. The interaction was confirmed by in vivo comimunoprecipitation and in vitro binding analysis. Neither interaction between Cyclin D1 and hATF5 nor interaction between Cyclin D2 and hATF5 was observed. Confocal microscopy analysis showed that Cyclin D3 could colocalize with hATF5 in the nuclear region. Cyclin D3 could potentiate hATF5 transcriptional activity independently of its Cdk4 partner. But Cyclin D1 and Cyclin D2 had no effect on hATF5 transcriptional activity. These data provide a new clue to understand the new role of Cyclin D3 as a transcriptional regulator. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：954 / 960

页数：7

共 33 条

[1] Cyclin D1 associates with the TBP-associated factor TAFII250 to regulate Sp1-mediated transcription [J].