Determination of proteins by fluorescence quenching of erythrosin B

被引：69

作者：

Ma, CQ ^{[1
]}

Li, KA ^{[1
]}

Tong, SY ^{[1
]}

机构：

[1] BEIJING UNIV,DEPT CHEM,BEIJING 100871,PEOPLES R CHINA

来源：

ANALYTICA CHIMICA ACTA | 1996年 / 333卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

fluorescence quenching; proteins; erythrosin B;

D O I：

10.1016/0003-2670(96)00280-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Erythrosin B (EB) binding to proteins causes a decrease in the fluorescence maximum of EB at 550 nm. Based on this, a new, fast and simple fluorescence quenching method for the determination of proteins is developed. The linear range of this assay is 1.36-20.4 mu g ml(-1). The method has very few interferences, most of which can be minimized by dilution. In the detection of proteins in human serum, this method gave values close to that of the Coomassie brilliant blue method.

引用

页码：83 / 88

页数：6

共 15 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

FASMAN GD, 1976, CRC PRACTICAL HDB BI, V2

[3] THE DETERMINATION OF A SMALL AMOUNT OF BIOLOGICAL CONSTITUENT BY THE USE OF CHEMI-LUMINESCENCE .13. HIGH SENSITIVE METAL CHELATE AFFINITY-CHROMATOGRAPHY [J].