Prevalence of Clostridium difficile in diarrhoeic and non-diarrhoeic piglets

被引:88
作者
Alvarez-Perez, Sergio [1 ]
Blanco, Jose L. [1 ]
Bouza, Emilio [2 ]
Alba, Patricia [1 ]
Gibert, Xavier [3 ]
Maldonado, Jaime [3 ]
Garcia, Marta E. [1 ]
机构
[1] UCM, Fac Vet, Dept Anim Hlth, Madrid 28040, Spain
[2] Hosp Gen Univ Gregorio Maranon, Div Clin Microbiol & Infect Dis, Madrid 28007, Spain
[3] Labs Hipra SA, Amer 17170, Girona, Spain
关键词
Clostridium difficile; Prevalence; Pig; Diarrhoea; Toxin; NEONATAL SWINE; FOOD ANIMALS; TOXIN-A; DISEASE; INFECTION; LOSSES; EUROPE; DOGS; PCR;
D O I
10.1016/j.vetmic.2009.01.015
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Clostridium difficile is considered to be an important causative agent of porcine neonatal diarrhoea, having taken over from classic bacteria] pathogens. However, there are currently no clear data concerning the prevalence of this microorganism in piglets, or about its relative distributions among diarrhoeic and non-diarrhoeic animals. In the present study, we analyzed the presence of C. difficile in rectal swabs from 780 piglets from two age groups (newborn and 1-2-month-old pigs) by means of molecular and microbiological procedures. Furthermore DNA was isolated from the bacteria in order to identify toxin A and toxin B genes. C difficile was not found in any of the 239 samples taken from 1- to 2-month-old pigs. Bacteria were, however, recovered from 140 out of 541 newborn piglets (25.9%), including both diarrhoeic and non-diarrhoeic animals, and animals from control farms (free of diarrhoeic animals). Genes for the production of both toxins A and B were identified in 132 of the 140 isolates (A(+)B(+)). Only seven isolates, all from the same farm and from non-diarrhoeic animals, lacked both toxin genes (A(-)B(-)), while one isolate from this same group of animals was A(-)B(+). This study provides the first report comparing the prevalence of C difficile in large numbers of diarrhoeic and non-diarrhoeic animals. There was no clear link between bacterial isolation and neonatal porcine diarrhoea. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:302 / 305
页数:4
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