Clostridium difficile is considered to be an important causative agent of porcine neonatal diarrhoea, having taken over from classic bacteria] pathogens. However, there are currently no clear data concerning the prevalence of this microorganism in piglets, or about its relative distributions among diarrhoeic and non-diarrhoeic animals. In the present study, we analyzed the presence of C. difficile in rectal swabs from 780 piglets from two age groups (newborn and 1-2-month-old pigs) by means of molecular and microbiological procedures. Furthermore DNA was isolated from the bacteria in order to identify toxin A and toxin B genes. C difficile was not found in any of the 239 samples taken from 1- to 2-month-old pigs. Bacteria were, however, recovered from 140 out of 541 newborn piglets (25.9%), including both diarrhoeic and non-diarrhoeic animals, and animals from control farms (free of diarrhoeic animals). Genes for the production of both toxins A and B were identified in 132 of the 140 isolates (A(+)B(+)). Only seven isolates, all from the same farm and from non-diarrhoeic animals, lacked both toxin genes (A(-)B(-)), while one isolate from this same group of animals was A(-)B(+). This study provides the first report comparing the prevalence of C difficile in large numbers of diarrhoeic and non-diarrhoeic animals. There was no clear link between bacterial isolation and neonatal porcine diarrhoea. (C) 2009 Elsevier B.V. All rights reserved.
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CDC, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Ctr Dis Control & Prevent, Atlanta, GA 30333 USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Jhung, Michael A.
;
Thompson, Angela D.
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Ctr Dis Control & Prevent, Atlanta, GA 30333 USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Thompson, Angela D.
;
Killgore, George E.
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Ctr Dis Control & Prevent, Atlanta, GA 30333 USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Killgore, George E.
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Zukowski, Walter E.
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Edward Hines Vet Adm Med Ctr, Chicago, IL USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Zukowski, Walter E.
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Songer, Glenn
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Univ Arizona, Tucson, AZ USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Songer, Glenn
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Warny, Michael
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Acambis Inc, Cambridge, MA USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Warny, Michael
;
Johnson, Stuart
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Edward Hines Vet Adm Med Ctr, Chicago, IL USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Johnson, Stuart
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Gerding, Dale N.
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Edward Hines Vet Adm Med Ctr, Chicago, IL USA
Loyola Univ, Stritch Sch Med, Chicago, IL 60611 USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Gerding, Dale N.
;
McDonald, L. Clifford
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Loyola Univ, Stritch Sch Med, Chicago, IL 60611 USA
Ctr Dis Control & Prevent, Atlanta, GA 30333 USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
McDonald, L. Clifford
;
Limbago, Brandi M.
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Ctr Dis Control & Prevent, Atlanta, GA 30333 USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
机构:
CDC, Div Healthcare Qual Promot, Atlanta, GA 30333 USA
Ctr Dis Control & Prevent, Atlanta, GA 30333 USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Jhung, Michael A.
;
Thompson, Angela D.
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Ctr Dis Control & Prevent, Atlanta, GA 30333 USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Thompson, Angela D.
;
Killgore, George E.
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Ctr Dis Control & Prevent, Atlanta, GA 30333 USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Killgore, George E.
;
Zukowski, Walter E.
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h-index: 0
机构:
Edward Hines Vet Adm Med Ctr, Chicago, IL USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Zukowski, Walter E.
;
Songer, Glenn
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h-index: 0
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Univ Arizona, Tucson, AZ USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Songer, Glenn
;
Warny, Michael
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Acambis Inc, Cambridge, MA USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Warny, Michael
;
Johnson, Stuart
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h-index: 0
机构:
Edward Hines Vet Adm Med Ctr, Chicago, IL USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Johnson, Stuart
;
Gerding, Dale N.
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h-index: 0
机构:
Edward Hines Vet Adm Med Ctr, Chicago, IL USA
Loyola Univ, Stritch Sch Med, Chicago, IL 60611 USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
Gerding, Dale N.
;
McDonald, L. Clifford
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h-index: 0
机构:
Loyola Univ, Stritch Sch Med, Chicago, IL 60611 USA
Ctr Dis Control & Prevent, Atlanta, GA 30333 USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA
McDonald, L. Clifford
;
Limbago, Brandi M.
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h-index: 0
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Ctr Dis Control & Prevent, Atlanta, GA 30333 USAEdward Hines Vet Adm Med Ctr, Chicago, IL USA