A new technique distinguishing α2-3 sialyl linkage from α2-6 linkage in sialyllactoses and sialyl-N-acetyllactosamines by post-source decay fragmentation method of MALDI-TOF mass spectrometry

被引：28

作者：

Yamagaki, T ^{[1
]}

Nakanishi, H ^{[1
]}

机构：

[1] Natl Inst Biosci & Human Technol, Tsukuba, Ibaraki 3058566, Japan

来源：

GLYCOCONJUGATE JOURNAL | 1999年 / 16卷 / 08期

关键词：

sialyl oligosaccharides; alpha 2-3/alpha 2-6 sialyl linkage analysis; MALDI-TOFMS; PSD fragmentation; new matrix;

D O I：

10.1023/A:1007050909293

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha 2-3 and alpha 2-6 sialyl linkage types of sialyllactoses and sialyl-N-acetyllactosamines were analyzed by post-source decay (PSD) fragmentation method using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. A new matrix of norharmane was suited for the MALDI-TOF measurements of sialyl oligosaccharides. The fragment ions B-1 produced by the cleavage of alpha 2-3 sialyl linkages indicate much higher intensity than those produced by the cleavage of alpha 2-6 sialyl linkages in sialyllactoses and sialyl-N-acetyllactosamines. Thus, alpha 2-3 sialyl linkages cleave much easier than alpha 2-6 sialyl linkages in MALDI-PSD fragmentation method. These results suggest that the new techniques using PSD fragmentation of MALDI-TOF mass spectrometry enables us to distinguish alpha 2-3 sialyl linkage from alpha 2-6 linkage in sialyl oligosaccharides.

引用

页码：385 / 389

页数：5

共 17 条

[1] Determination of the sialylation level and of the ratio α-(2→3)/α-(2→6) sialyl linkages of N-glycans by methylation and GC/MS analysis [J].

Cointe, D ;

Leroy, C ;

Chirat, F .

CARBOHYDRATE RESEARCH, 1998, 311 (1-2) :51-59

[2] A CURVED FIELD REFLECTRON TIME-OF-FLIGHT MASS-SPECTROMETER FOR THE SIMULTANEOUS FOCUSING OF METASTABLE PRODUCT IONS [J].

CORNISH, TJ ;

COTTER, RJ .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1994, 8 (09) :781-785

[3] A SYSTEMATIC NOMENCLATURE FOR CARBOHYDRATE FRAGMENTATIONS IN FAB-MS MS SPECTRA OF GLYCOCONJUGATES [J].

DOMON, B ;

COSTELLO, CE .

GLYCOCONJUGATE JOURNAL, 1988, 5 (04) :397-409

[4] COLLISIONAL-ACTIVATION TANDEM MASS-SPECTROMETRY OF SODIUM ADDUCT IONS OF METHYLATED OLIGOSACCHARIDES - SEQUENCE-ANALYSIS AND DISCRIMINATION BETWEEN ALPHA-NEUAC-(2-]3) AND ALPHA-NEUAC-(2-]6) LINKAGES [J].

LEMOINE, J ;

STRECKER, G ;

LEROY, Y ;

FOURNET, B ;

RICART, G .

CARBOHYDRATE RESEARCH, 1991, 221 :209-217

[5]

Nonami H, 1998, RAPID COMMUN MASS SP, V12, P285, DOI 10.1002/(SICI)1097-0231(19980331)12:6<285::AID-RCM158>3.0.CO

[6]

2-4

[7]

Nonami H, 1997, J MASS SPECTROM, V32, P287, DOI 10.1002/(SICI)1096-9888(199703)32:3<287::AID-JMS478>3.0.CO

[8]

2-#

[9] Analysis of acidic oligosaccharides and glycopeptides by matrix assisted laser desorption ionization time-of-flight mass spectrometry [J].

Papac, DI ;

Wong, A ;

Jones, AJS .

ANALYTICAL CHEMISTRY, 1996, 68 (18) :3215-3223

[10] SIALIDASE OF SWINE INFLUENZA-A VIRUSES - VARIATION OF THE RECOGNITION SPECIFICITIES FOR SIALYL LINKAGES AND FOR THE MOLECULAR-SPECIES OF SIALIC-ACID WITH THE YEAR OF ISOLATION [J].

XU, GY ;

SUZUKI, T ;

MAEJIMA, Y ;

MIZOGUCHI, T ;

TSUCHIYA, M ;

KISO, M ;

HASEGAWA, A ;

SUZUKI, Y .

GLYCOCONJUGATE JOURNAL, 1995, 12 (02) :156-161

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