Synthesis and properties of amphiphilic networks.: 1:: the effect of hydration and polymer composition on the adhesion of immunoglobulin-G to poly(laurylmethacrylate-stat-glycerolmonomethacrylate-stat-ethylene-glycol-dimethacrylate) networks

被引:45
作者
Haigh, R
Rimmer, S
Fullwood, NJ [1 ]
机构
[1] Univ Lancaster, Dept Biol Sci, Lancaster LA1 4YQ, England
[2] Univ Lancaster, Ctr Polymer, Lancaster LA1 4YA, England
基金
英国工程与自然科学研究理事会;
关键词
adsorption; IgG; hydration; hydrogels;
D O I
10.1016/S0142-9612(99)00245-8
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
A series of hydrogels composed of varying fractions of dodecyl methacrylate (DM) and 2,3-dihydroxypropyl methacrylate (GM) were prepared using ethylene glycol dimethacrylate (EGDMA) as the cross-linking agent. The study found that for a series of gels with the same monomer ratio, bulk hydration could be controlled by adjusting the cross-link density. The ability to control cross-link density allowed the preparation of gels with the same bulk hydration but different ratios of the two monomers. The adsorption of IgG to the gels was investigated using ELISA. The aim of the project was to investigate the effect of the bulk hydration and polymer composition on IgG adsorption. The results show that for a series of gels with the same monomer ratio, there is a clear trend towards a reduction in protein adsorption as the bulk hydration and accompanying chain mobility of the gel increases. Studies on gels of the same bulk hydration but differing ratios of monomer show higher protein adsorption as the proportion of GM increases. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:735 / 739
页数:5
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