Use of a single, triplicate arbitrarily primed-PCR procedure for molecular fingerprinting of lactic acid bacteria

被引：42

作者：

Cusick, SM

O'Sullivan, DJ

机构：

[1] Univ Minnesota, Dept Food Sci & Nutr, St Paul, MN 55108 USA

[2] Univ Minnesota, Dept Microbial Engn, St Paul, MN 55108 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 05期

关键词：

D O I：

10.1128/AEM.66.5.2227-2231.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Arbitrarily primed (AP)-PCR can be used to generate characteristic DNA fingerprint patterns. However, small changes in reaction conditions can cause band irreproducibility. In this study, a single methodology encompassing triplicate reactions, which were intentionally exposed to three different annealing temperatures, enabled bands that were reproducibly generated to be recognized. A single triplicate AP-PCR (TAP-PCR) procedure, using an Ig-mer primer, was developed and used to fingerprint representative isolates from the major genera of lactic acid bacteria and Bifidobacterium to the strain level.

引用

页码：2227 / 2231

页数：5

共 18 条

[1]

BASSAM BJ, 1992, APPL MICROBIOL BIOT, V38, P70, DOI 10.1007/BF00169422

[2] Classification and identification of bacteria: Current approaches to an old problem. Overview of methods used in bacterial systematics [J].