Senescence-like changes induced by expression of p21Waf1/Cip1 in NIH3T3 cell line

被引:41
作者
Chen, X
Zhang, W
Gao, YF
Su, XQ
Zhai, ZH [1 ]
机构
[1] Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China
[2] Beijing Normal Univ, Coll Life Sci, Key Lab Cell Proliferat & Regulat Biol, Minist Educ, Beijing 100875, Peoples R China
关键词
p21(Waf1/Cip1); senescence; inducible expression; cell cycle arrest;
D O I
10.1038/sj.cr.7290129
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
P21(Waf1/Cip1) is a potent cyclin-dependent kinase inhibitor. As a downstream mediator of p53, p21(Waf1/Cip1) involves in cell cycle arrest, differentiation and apoptosis. Previous studies in human cells provided evidence for a link between p21(Waf1/Cip1) and cellular senescence. While in murine cells, the role of p21(Waf1/Cip1) is indefinite. We explored this issue using NIH3T3 cells with inducible p21(Waf1/Cip1) expression. Induction of p21(Waf1/Cip1) triggered G1 growth arrest, and NIH3T3-p21 cells exhibited morphologic features, such as enlarged and flattened cellular shape, specific to the senescence phenotype. We also showed that p21(Waf1/Cip1)-transduced NIH3T3 cells expressed beta-galactosidase activity at pH 6.0, which is known to be a marker of senescence. Our results suggest that p21(Waf1/Cip1) can also induce senescence-like changes in murine cells.
引用
收藏
页码:229 / 233
页数:5
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