Characterization of air plasma-treated polymer surfaces by ESCA and contact angle measurements for optimization of surface stability and cell growth

被引:63
作者
Johansson, BL
Larsson, A [1 ]
Ocklind, A
Öhrlund, Å
机构
[1] Amersham Biosci AB, R&D, SE-75184 Uppsala, Sweden
[2] Gyros AB, SE-75183 Uppsala, Sweden
[3] Melacure Therapeut, SE-75643 Uppsala, Sweden
[4] Pharmacia Corp, SE-75182 Uppsala, Sweden
关键词
biomaterials; cold plasma; ESCA/XPS; surfaces;
D O I
10.1002/app.11209
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
A radiofrequency air plasma has been used to incorporate new functionalities at the surface of cycloolefin polymers (Zeonex(R) and Topas(R)), polymethyl methacrylate (PMMA), styrene-acrylonitrile copolymer (SAN), and polystyrene (PS). The main goals with the plasma treatment of the different plastics were to hydrophilize the surfaces and to provide good cell culture properties. Surfaces treated at high RF power/gas flow ratios (50 to 100 W/sccm) became highly hydrophilic (water contact angles of about 5 degrees) and stable towards washing in 70% (v/v) ethanol. Those treated at lower power/gas flow ratios (3 to 10 W/sccm) were less hydrophilic and not wash-stable. Cell growth properties of HeLa cervix carcinoma cells as good as on commercial tissue-culture polystyrene could be obtained for Zeonex, SAN, and PS, treated at relatively low RF power/gas flow ratios. However, no untreated plastics were suitable for culturing these cells. XPS spectra features show that ester, ether/alcohol, and ester/carboxyl groups are formed during the plasma treatments of the different plastics. Measurable amounts of carboxylic acid carbon after plasma treatment were only observed for PS and Topas. Furthermore, at high RF power/gas flow ratios fluorine, aluminium and silicon were incorporated in all investigated plastics surfaces due to ablation-deposition processes in the reaction chamber. (C) 2002 Wiley Periodicals, Inc.
引用
收藏
页码:2618 / 2625
页数:8
相关论文
共 23 条
[1]  
Beamson G., 1992, ADV MATER, DOI DOI 10.1002/ADMA.19930051035
[2]  
Chinn J. A., 1994, Journal of Tissue Culture Methods, V16, P155, DOI 10.1007/BF01540643
[3]  
Denes F., 1997, J. Photopolym. Sci. Technol, V10, P91
[4]  
Fozza A., 1999, PLASMAS POLYM, V4, P183, DOI DOI 10.1023/A:1021853026619
[5]   Non-isothermal O-2 plasma treatment of phenyl-containing polymers [J].
Greenwood, OD ;
Hopkins, J ;
Badyal, JPS .
MACROMOLECULES, 1997, 30 (04) :1091-1098
[6]  
Klemberg-Sapieha JE, 1999, MATER RES SOC SYMP P, V544, P277
[7]   Stability of polycarbonate and polystyrene surfaces after hydrophilization with high intensity oxygen RF plasma [J].
Larsson, A ;
Dérand, H .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2002, 246 (01) :214-221
[8]  
Larsson A., 2000, P 2 INT S POLYM SURF, P121
[9]   SECONDARY-ION MASS-SPECTROMETRY TIME-OF-FLIGHT AND IN-SITU X-RAY PHOTOELECTRON-SPECTROSCOPY STUDIES OF POLYMER SURFACE MODIFICATIONS BY A REMOTE OXYGEN PLASMA TREATMENT [J].
LIANOS, L ;
PARRAT, D ;
HOC, TQ ;
DUC, TM .
JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS, 1994, 12 (04) :2491-2498
[10]   NEAR-UV RADIATION-INDUCED SURFACE GRAFT-COPOLYMERIZATION OF SOME O-3-PRETREATED CONVENTIONAL POLYMER-FILMS [J].
LOH, FC ;
TAN, KL ;
KANG, ET ;
NEOH, KG ;
PUN, MY .
EUROPEAN POLYMER JOURNAL, 1995, 31 (05) :481-488