Genes expressed in sugarcane maturing internodal tissue

被引：56

作者：

Carson, DL

Botha, FC

机构：

[1] S African Sugar Assoc Expt Stn, Dept Biotechnol, ZA-4300 Mt Edgecombe, South Africa

[2] Univ Stellenbosch, Inst Plant Biotechnol, ZA-7602 Matieland, South Africa

来源：

PLANT CELL REPORTS | 2002年 / 20卷 / 11期

关键词：

expressed sequence tags; cDNA; gene expression;

D O I：

10.1007/s00299-002-0444-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

To explore gene expression during sugarcane culm maturation, we performed a partial sequence analysis of random clones from maturing culm total and subtracted cDNA libraries. Database comparisons revealed that of the 337 cDNA sequences analysed, 167 showed sequence homology to gene products in the protein databases, while 111 matched uncharacterised plant expressed sequence tags (ESTs) only. The remaining cDNAs showed no database match and could represent novel genes. The majority of ESTs corresponded to a variety of genes associated with general cellular metabolism. ESTs homologous to various stress response genes were also well represented. Analysis of ESTs from the subtracted library identified genes that may be preferentially expressed during culm maturation. This research has provided a framework for functional gene analysis in sugarcane sucrose-accumulating tissues.

引用

页码：1075 / 1081

页数：7

共 25 条

[1] Analysis of grape ESTs: global gene expression patterns in leaf and berry [J].