Double-stranded RNA and virus-like particles in the edible basidiomycete Flammulina velutipes (Enokitake)

被引：32

作者：

Magae, Y

Hayashi, N

机构：

[1] Forestry & Forest Prod Res Inst, Bioresources Technol Div, Tsukuba, Ibaraki 3058687, Japan

[2] Forestry & Forest Prod Res Inst, Wood Chem Div, Tsukuba, Ibaraki 3058687, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 1999年 / 180卷 / 02期

关键词：

double-stranded RNA; virus-like particle; degeneration; Flammulina velutipes;

D O I：

10.1111/j.1574-6968.1999.tb08814.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

One of the commercial strains of Flammulina velutipes was analyzed for the presence of double-stranded RNA (dsRNA) elements to examine the underlying mechanism of strain degeneration. As a result, two dsRNA elements sized 1.9 and 1.8 kb were detected in mycelium derived from spontaneously brown-colored fruit body. They were not detected in the normal strains or in fruiting-impaired degenerative isolates. The dsRNAs were not in the nuclear or mitochondrial fractions, but were located in the cytoplasmic fraction. The presence of virus-like particles of ca. 50 nm diameter associated with the dsRNAs was confirmed by electron microscopic observation. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：331 / 335

页数：5

共 11 条

[1]

BABASAKI K, 1999, MAFF MICROORG GENET, V5, P23

[2] EVIDENCE FOR VIRAL AND NAKED DOUBLE-STRANDED RNAS IN THE BASIDIOMYCETE AGROCYBE-AEGERITA [J].