Myosin heavy chains in fibers of TTX-paralyzed rat soleus and medial gastrocnemius muscles

被引:13
作者
Cormery, B
Pons, F
Marini, JF
Gardiner, PF
机构
[1] Univ Montreal, Dept Kinesiol, Montreal, PQ H3C 3J7, Canada
[2] INSERM U300, F-34060 Montpellier, France
[3] Ctr Natl Rech Sci, Unite Neurobiol Cellulaire, F-13007 Marseille, France
关键词
fiber types; tetrodotoxin; compartmentalization; antibodies;
D O I
10.1152/jappl.2000.88.1.66
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The expression of five myosin heavy chain (MHC) isoforms was analyzed in the rat soleus (Sol) and the deep and superficial medial gastrocnemius (dGM, sGM) muscle after 2 and 4 wk of TTX paralysis by using immunohistochemical techniques. In Sol, after 4 wk of paralysis, fibers containing type I MHC were either pure type I (14%) or also contained developmental (D; 76%), IIa (26%), or IIx (18%) MHC. Values for corresponding fibers in dGM were 8.5, 65, 38, and 22%. Also, by 4 wk an increase was seen in the proportions of fibers expressing IIa MHC in Sol (from 16 to 38%) and dGM (from 24 to 74%). In a region of sGM in control muscles containing pure IIb fibers, a major proportion (86%) remained pure after 4 wk of paralysis, with the remainder coexpressing IIb and IIx. The results indicate that TTX-induced muscle paralysis results in an increase in fibers containing multiple MHC isoforms and that the D isoform appears in a major proportion of these hybrid fibers.
引用
收藏
页码:66 / 76
页数:11
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