Characterization of novel cysteine-rich antimicrobial peptides from scorpion blood

被引:176
作者
EhretSabatier, L
Loew, D
Goyffon, M
Fehlbaum, P
Hoffmann, JA
vanDorsselaer, A
Bulet, P
机构
[1] INST BIOL MOL & CELLULAIRE,UPR 9022 CNRS,F-67084 STRASBOURG,FRANCE
[2] UNIV STRASBOURG 1,FAC CHIM,LAB SPECTROMETRIE MASSE BIOORGAN,URA 31 CNRS,F-67008 STRASBOURG,FRANCE
[3] MUSEUM NATL HIST NAT,F-75005 PARIS,FRANCE
[4] DIV BIOL GEN & ECOL,LAB ETUD & RECH ARTHROPODES IRRADIES,F-75005 PARIS,FRANCE
关键词
D O I
10.1074/jbc.271.47.29537
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated, from the hemolymph of unchallenged scorpions of the species Androctonus australis, three distinct antimicrobial peptides, which we have fully characterized by Edman degradation, electrospray ionization mass spectrometry, and matrix-assisted laser desorption/ionization mass spectrometry. Two are novel molecules: (i) androctonin, a 25-residue peptide with two disulfide bridges, active against both bacteria (Gram-positive and Gram-negative) and fungi and showing marked sequence homology to tachyplesins and polyphemusins from horseshoe crabs; and (ii) buthinin, a 34-residue antibacterial (Gram-positive and Gram-negative) peptide with three disulfide bridges. The third peptide contains 37 residues and three disulfide bridges and clearly belongs to the family of anti-Gram-positive insect defensins, We have synthesized androctonin and explored its activity spectrum and mode of action.
引用
收藏
页码:29537 / 29544
页数:8
相关论文
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