Method based on solid-phase microextraction-high-performance liquid chromatography with UV and electrochemical detection to determine estrogenic compounds in water samples

被引：129

作者：

Peñalver, A ^{[1
]}

Pocurull, E ^{[1
]}

Borrull, F ^{[1
]}

Marcé, RM ^{[1
]}

机构：

[1] Univ Rovira & Virgili, Dept Quim Analit & Quim Organ, Tarragona 43005, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 964卷 / 1-2期

关键词：

water analysis; environmental analysis; endocrine disruptors; estrogenic compounds;

D O I：

10.1016/S0021-9673(02)00694-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We determined a group of estrogenic compounds by solid-phase microextraction (SPME) coupled to high-performance liquid chromatography (HPLC) with both ultraviolet (UV) and electrochemical detection (ED). A modified liquid chromatograph was used. Polyacrylate fibers (85 km) were used to extract the analytes from the aqueous samples. Dynamic and static modes of desorption were compared and the variables affecting both absorption and desorption processes in SPME-HPLC were optimized. Static desorption gave the best recoveries and peak shapes. The performance of the SPME-HPLC-UV-ED method was checked with river water and wastewater. The method enabled estrogenic compounds to be determined at low-mug l(-1) levels in real water samples. Limits of detection were between 0.3 and 1.1 mug l(-1) using UV detection and between 0.06 and 0.08 mug l(-1) using ED. P-Estradiol was found in samples from a wastewater treatment plant at concentrations between 1.9 and 2.2 mug l(-1). (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：153 / 160

页数：8

共 21 条

[1] Analysis and occurrence of estrogenic hormones and their glucuronides in surface water and waste water in The Netherlands [J].

Belfroid, AC ;

Van der Horst, A ;

Vethaak, AD ;

Schäfer, AJ ;

Rijs, GBJ ;

Wegener, J ;

Cofino, WP .

SCIENCE OF THE TOTAL ENVIRONMENT, 1999, 225 (1-2) :101-108

[2] Solid-phase microextraction coupled with high-performance liquid chromatography for the determination of alkylphenol ethoxylate surfactants in water [J].

BoydBoland, AA ;

Pawliszyn, JB .

ANALYTICAL CHEMISTRY, 1996, 68 (09) :1521-1529

[3] Analysis of industrial effluents to determine endocrine-disrupting chemicals [J].

Castillo, M ;

Barcelo, D .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1997, 16 (10) :574-583

[4] Determination of steroid sex hormones and related synthetic compounds considered as endocrine disrupters in water by fully automated on-line solid-phase extraction-liquid chromatography-diode array detection [J].

de Alda, MJL ;

Barceló, D .

JOURNAL OF CHROMATOGRAPHY A, 2001, 911 (02) :203-210

[5] Determination of steroid sex hormones and related synthetic compounds considered as endocrine disrupters in water by liquid chromatography-diode array detection-mass spectrometry [J].

de Alda, MJL ;

Barceló, D .

JOURNAL OF CHROMATOGRAPHY A, 2000, 892 (1-2) :391-406

[6] Determination of steroid estrogens in wastewater by immunoaffinity extraction coupled with HPLC-Electrospray-MS [J].

Ferguson, PL ;

Iden, CR ;

McElroy, AE ;

Brownawell, BJ .

ANALYTICAL CHEMISTRY, 2001, 73 (16) :3890-3895

[7] Gas chromatographic-mass spectrometric method for separation and detection of endocrine disruptors from environmental water samples [J].

Helaleh, MIH ;

Takabayashi, Y ;

Fujii, S ;

Korenaga, T .

ANALYTICA CHIMICA ACTA, 2001, 428 (02) :227-234

[8] The power of analytical methods for measuring suspected endocrine disrupting compounds: a pilot field study [J].

Jones-Lepp, T ;

Gerlach, CL ;

Cooter, EJ .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2000, 19 (05) :286-291

[9]

Long GL, 1983, ANAL CHEM, V55, p712A, DOI [DOI 10.1021/AC00258A001, 10.1021/ac00258a001]

[10] Environmental exposure, species differences and risk assessment [J].

Miyamoto, J ;

Klein, W .

PURE AND APPLIED CHEMISTRY, 1998, 70 (09) :1829-1845

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