Determination of hydroxycinnamic acids, hydroxybenzoic acids, hydroxybenzaldehydes, hydroxybenzyl alcohols and their glucosides by high-performance liquid chromatography

Three 4-hydroxycinnamic acids, three 4-hydroxybenzoic acids, three 4-hydroxybenzaldehydes, three 4-hydroxybenzyl alcohols and their glucosides were separated by HPLC. HPLC analyses were performed with an octadecyl silica gel column and a gradient elution with methanol and water adjusted to pH 4.6 or 3.1. Compounds extracted from Vanilla planifolia green beans were analyzed by this method and 4-hydroxybenzaldehyde, vanillin and their respective glucosides were determined.