Relative abundances, isolation and structure of phlebotomine microsatellites

被引:13
作者
Day, JC [1 ]
Ready, PD [1 ]
机构
[1] Nat Hist Museum, Dept Entomol, Mol Systemat Lab, London SW7 5BD, England
关键词
microsatellites; AAT-class repeats; Lutzomyia whitmani; phlebotomine sandflies; website;
D O I
10.1046/j.1365-2583.1999.00142.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Popular classes of microsatellites are not always abundant in insects or easily isolated from them. Dot-blot hybridizations demonstrated much variation in the relative abundance of four repeal: classes in four phlebotomine sandfly species. Only AAT-class repeats were specifically isolated from a phagemid library of Lutzomyia whitmani, even though other microsatellites had similar abundances. An enrichment step would have targeted classes but was omitted because relatively long flanking sequences were sought. All fourteen sandfly loci had a non-coding structure, and a minority of dipteran AAT-class repeats found in DNA databases and the literature were from exons, Therefore, this class should often provide neutral alleles for population studies. Perfect, not imperfect, AAT-class repeats were polymorphic in wild L. whitmani.
引用
收藏
页码:575 / 580
页数:6
相关论文
共 20 条
[1]   REASSOCIATION KINETICS OF ANOPHELES-GAMBIAE (DIPTERA, CULICIDAE) DNA [J].
BESANSKY, NJ ;
POWELL, JR .
JOURNAL OF MEDICAL ENTOMOLOGY, 1992, 29 (01) :125-128
[2]  
BOOTH DR, 1993, THESIS U LONDON
[3]   COLONY BANK CONTAINING SYNTHETIC COL EL HYBRID PLASMIDS REPRESENTATIVE OF ENTIRE ESCHERICHIA-COLI GENOME [J].
CLARKE, L ;
CARBON, J .
CELL, 1976, 9 (01) :91-99
[4]   Evolution of simple repeats in DNA and their relation to human disease [J].
Djian, P .
CELL, 1998, 94 (02) :155-160
[5]  
Hammond Robert L., 1998, P279
[6]   Microsatellites, from molecules to populations and back [J].
Jarne, P ;
Lagoda, PJL .
TRENDS IN ECOLOGY & EVOLUTION, 1996, 11 (10) :424-429
[7]   PHLEBOTOMINE VECTORS OF THE LEISHMANIASES - A REVIEW [J].
KILLICKKENDRICK, R .
MEDICAL AND VETERINARY ENTOMOLOGY, 1990, 4 (01) :1-24
[8]  
Knudson Dennis L., 1996, P175
[9]   BRAIN-CELL KARYOTYPES OF 6 SPECIES OF NEW AND OLD-WORLD SAND FLIES (DIPTERA, PSYCHODIDAE) [J].
KREUTZER, RD ;
MODI, GB ;
TESH, RB ;
YOUNG, DG .
JOURNAL OF MEDICAL ENTOMOLOGY, 1987, 24 (06) :609-612
[10]   Microsatellite sequences are under-represented in two mite genomes [J].
Navajas, MJ ;
Thistlewood, HMA ;
Lagnel, J ;
Hughes, C .
INSECT MOLECULAR BIOLOGY, 1998, 7 (03) :249-256