The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of Crumbs and Discs Lost

被引:302
作者
Roh, MH
Makarova, O
Liu, CJ
Shin, K
Lee, S
Laurinec, S
Goyal, M
Wiggins, R
Margolis, B
机构
[1] Univ Michigan, Sch Med, Dept Biol Chem, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Sch Med, Howard Hughes Med Inst, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Sch Med, Dept Internal Med, Ann Arbor, MI 48109 USA
关键词
Maguk; PDZ domain; Discs Lost; stardust; Crumbs;
D O I
10.1083/jcb.200109010
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Membrane-associated guanylate kinase (Maguk) proteins are scaffold proteins that contain PSD-95-Discs Large-zona occludens-1 (PDZ), Src homology 3, and guanylate kinase domains. A subset of Maguk proteins, such as mLin-2 and protein associated with Lin-7 (Pals)1, also contain two L27 domains: an L27C domain that binds mLin-7 and an L27N domain of unknown function. Here, we demonstrate that the L27N domain targets Pals1 to tight junctions by binding to a PDZ domain protein, Pals1-associated tight junction (PAT) protein, via a unique Maguk recruitment domain. PATJ is a homologue of Drosophila Discs Lost, a protein that is crucial for epithelial polarity and that exists in a complex with the apical polarity determinant, Crumbs. PATJ and a human Crumbs homologue, CRB1, colocalize with Pals1 to tight junctions, and CRB1 interacts with PATJ albeit indirectly via binding the Pals1 PDZ domain. In agreement, we find that a Drosophila homologue of Pals1 participates in identical interactions with Drosophila Crumbs and Discs Lost. This Drosophila Pals1 homologue has been demonstrated recently to represent Stardust, a crucial polarity gene in Drosophila. Thus, our data identifies a new multiprotein complex that appears to be evolutionarily conserved and likely plays an important role in protein targeting and cell polarity.
引用
收藏
页码:161 / 172
页数:12
相关论文
共 44 条
[1]   Drosophila Stardust is a partner of Crumbs in the control of epithelial cell polarity [J].
Bachmann, A ;
Schneider, M ;
Theilenberg, E ;
Grawe, F ;
Knust, E .
NATURE, 2001, 414 (6864) :638-643
[2]   Discs lost, a novel multi-PDZ domain protein, establishes and maintains epithelial polarity [J].
Bhat, MA ;
Izaddoost, S ;
Lu, Y ;
Cho, KO ;
Choi, KW ;
Bellen, HJ .
CELL, 1999, 96 (06) :833-845
[3]   Cooperative regulation of cell polarity and growth by Drosophila tumor suppressors [J].
Bilder, D ;
Li, M ;
Perrimon, N .
SCIENCE, 2000, 289 (5476) :113-116
[4]  
Borg JP, 1999, J NEUROSCI, V19, P1307
[5]   Identification of an evolutionarily conserved heterotrimeric protein complex involved in protein targeting [J].
Borg, JP ;
Straight, SW ;
Kaech, SM ;
de Taddeo-Borg, M ;
Kroon, DE ;
Karnak, D ;
Turner, RS ;
Kim, SK ;
Margolis, B .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (48) :31633-31636
[6]   Zonula adherens formation in Caenorhabditis elegans requires dlg-1, the homologue of the Drosophila gene discs large [J].
Bossinger, O ;
Klebes, A ;
Segbert, C ;
Theres, C ;
Knust, E .
DEVELOPMENTAL BIOLOGY, 2001, 230 (01) :29-42
[7]   A tripartite protein complex with the potential to couple synaptic vesicle exocytosis to cell adhesion in brain [J].
Butz, S ;
Okamoto, M ;
Südhof, TC .
CELL, 1998, 94 (06) :773-782
[8]   A kinase-regulated PDZ-domain interaction controls endocytic sorting of the β2-adrenergic receptor [J].
Cao, TT ;
Deacon, HW ;
Reczek, D ;
Bretscher, A ;
von Zastrow, M .
NATURE, 1999, 401 (6750) :286-290
[9]   Human CASK/LIN-2 binds syndecan-2 and protein 4.1 and localizes to the basolateral membrane of epithelial cells [J].
Cohen, AR ;
Wood, DF ;
Marfatia, SM ;
Walther, Z ;
Chishti, AH ;
Anderson, JM .
JOURNAL OF CELL BIOLOGY, 1998, 142 (01) :129-138
[10]   Mutations in a human homologue of Drosophila crumbs cause retinitis pigmentosa (RP12) [J].
den Hollander, AI ;
ten Brink, JB ;
de Kok, YJM ;
van Soest, S ;
van den Born, LI ;
van Driel, MA ;
van de Pol, DJR ;
Payne, AM ;
Bhattacharya, SS ;
Kellner, U ;
Hoyng, CB ;
Westerveld, A ;
Brunner, HG ;
Bleeker-Wagemakers, EM ;
Deutman, AF ;
Heckenlively, JR ;
Cremers, FPM ;
Bergen, AAB .
NATURE GENETICS, 1999, 23 (02) :217-221