Use of a granular bioplastic formulation for carrying conidia of a non-aflatoxigenic strain of Aspergillus flavus

被引:37
作者
Accinelli, Cesare [1 ]
Sacca, M. Ludovica [1 ]
Abbas, Hamed K. [2 ]
Zablotowicz, Robert M. [3 ]
Wilkinson, Jeffery R. [4 ]
机构
[1] Univ Bologna, Dept Agroenvironm Sci & Technol, I-40127 Bologna, Italy
[2] USDA ARS, Crop Genet & Prod Res Unit, Stoneville, MS 38776 USA
[3] USDA ARS, So Weed Sci Unit, Stoneville, MS 38776 USA
[4] Mississippi State Univ, Dept Biochem & Mol Biol, Starkville, MS 39762 USA
关键词
Biocontrol formulations; Bioplastic; Mycotoxins; Biopesticides; RHIZOCTONIA-SOLANI; BIOLOGICAL-CONTROL; BIOSYNTHETIC-PATHWAY; TRICHODERMA; FUNGI; BIOCONTROL; GROWTH; CORN; SOIL; QUANTIFICATION;
D O I
10.1016/j.biortech.2009.03.010
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Previous research demonstrated that aflatoxin contamination in corn is reduced by field application of wheat grains pre-inoculated with the non-aflatoxigenic Aspergillus flavus strain NRRL 30797. To facilitate field applications of this biocontrol isolate, a series of laboratory studies were conducted on the reliability and efficiency of replacing wheat grains with the novel bioplastic formulation Mater-Bi (R) to serve as a carrier matrix to formulate this fungus. Mater-Bi (R) granules were inoculated with a conidial suspension of NRRL 30797 to achieve a final cell density of approximately log 7 conidia/granule. Incubation of 20-g soil samples receiving a single Mater-Bi (R) granule for 60-days resulted in log 4.2-5.3 propagules of A.flavus/g soil in microbiologically active and sterilized soil, respectively. Increasing the number of granules had no effect on the degree of soil colonization by the biocontrol fungus. In addition to the maintenance of rapid vegetative growth and colonization of soil samples, the bioplastic formulation was highly stable, indicating that Mater-Bi (R) is a suitable substitute for biocontrol applications of A. flavus NRRL 30797. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3997 / 4004
页数:8
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