Quantitative 3D Video Microscopy of HIV Transfer Across T Cell Virological Synapses

被引：385

作者：

Huebner, Wolfgang ^{[1
]}

McNerney, Gregory P. ^{[3
]}

Chen, Ping ^{[1
]}

Dale, Benjamin M. ^{[1
]}

Gordon, Ronald E. ^{[2
]}

Chuang, Frank Y. S. ^{[3
]}

Li, Xiao-Dong ^{[4
]}

Asmuth, David M. ^{[4
]}

Huser, Thomas ^{[3
,4
]}

Chen, Benjamin K. ^{[1
]}

机构：

[1] Mt Sinai Sch Med, Dept Med, Inst Immunol, Div Infect Dis, New York, NY 10029 USA

[2] Mt Sinai Sch Med, Dept Pathol, New York, NY 10029 USA

[3] Univ Calif Davis, NSF Ctr Biophoton Sci & Technol, Sacramento, CA 95817 USA

[4] Univ Calif Davis, Med Ctr, Dept Internal Med, Sacramento, CA 95817 USA

来源：

SCIENCE | 2009年 / 323卷 / 5922期

关键词：

IMMUNODEFICIENCY-VIRUS TYPE-1; GP41 CYTOPLASMIC TAIL; LYMPHOCYTES; FUSION; SPREAD; ENTRY; CYTOSKELETON; REPLICATION; ENDOCYTOSIS; INFECTION;

D O I：

10.1126/science.1167525

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized "buttons" containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.

引用

页码：1743 / 1747

页数：5

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