Methylglyoxal, protein binding and biological samples: Are we getting the true measure?

被引：80

作者：

Dhar, Arti ^{[1
]}

Desai, Kaushik ^{[1
]}

Liu, Jianghai ^{[1
]}

Wu, Lingyun ^{[1
]}

机构：

[1] Univ Saskatchewan, Dept Pharmacol, Coll Med, Saskatoon, SK S7N 5E5, Canada

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 11-12期

基金：

加拿大健康研究院;

关键词：

Methylglyoxal; Perchloric acid; o-Phenylenediamine; HPLC; PEROXYNITRITE PRODUCTION; CHEMICAL-MODIFICATION; GLYOXALASE SYSTEM; PLASMA; CELLS; DERIVATIZATION; PRODUCT; ASSAY; RATS; AGE;

D O I：

10.1016/j.jchromb.2009.02.055

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Methylglyoxal (MG), a reactive metabolic byproduct and a precursor of advanced glycation endproducts (AGES), is elevated in diabetes. In the body MG is free or reversibly or irreversibly bound (mostly with proteins). Variable plasma MG values have been reported. MG is commonly measured using high performance liquid chromatography. We tested several protocols on different biological samples, which resulted in significant differences in MG values measured in a given sample. The different values do not appear due to the release and detection of bound MG under assay conditions. Protocols that provide consistent values of MG in biological samples are recommended. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1093 / 1100

页数：8

共 32 条

[1]

N-epsilon-(carboxyethyl)lysine, a product of the chemical modification of proteins by methylglyoxal, increases with age in human lens proteins [J].

Ahmed, MU ;

Frye, EB ;

Degenhardt, TP ;

Thorpe, SR ;

Baynes, JW .

BIOCHEMICAL JOURNAL, 1997, 324 :565-570

[2]

Chromatographic assay of glycation adducts in human serum albumin glycated in vitro by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and intrinsic fluorescence [J].

Ahmed, N ;

Thornalley, PJ .

BIOCHEMICAL JOURNAL, 2002, 364 (01) :15-24

[3]

A METHOD FOR THE DETERMINATION OF ALPHA-DICARBONYL COMPOUNDS [J].

BEDNARSKI, W ;

JEDRYCHOWSKI, L ;

HAMMOND, EG ;

NIKOLOV, ZL .

JOURNAL OF DAIRY SCIENCE, 1989, 72 (10) :2474-2477

[4]

Metformin reduces systemic methylglyoxal levels in type 2 diabetes [J].

Beisswenger, PJ ;

Howell, SK ;

Touchette, AD ;

Lal, S ;

Szwergold, BS .

DIABETES, 1999, 48 (01) :198-202

[5]

NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase from Thermoproteus tenax -: The first identified archaeal, member of the aldehyde dehydrogenase superfamily is a glycolytic enzyme with unusual regulatory properties [J].

Brunner, NA ;

Brinkmann, H ;

Siebers, B ;

Hensel, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (11) :6149-6156

[6]

Methylglyoxal-induced nitric oxide and peroxynitrite production in vascular smooth muscle cells [J].

Chang, TJ ;

Wang, R ;

Wu, LY .

FREE RADICAL BIOLOGY AND MEDICINE, 2005, 38 (02) :286-293

[7]

Effect of endogenous methylglyoxal on Chinese hamster ovary cells grown in culture [J].

Chaplen, FWR ;

Fahl, WE ;

Cameron, DC .

CYTOTECHNOLOGY, 1996, 22 (1-3) :33-42

[8]

Evidence of high levels of methylglyoxal in cultured Chinese hamster ovary cells [J].

Chaplen, FWR ;

Fahl, WE ;

Cameron, DC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (10) :5533-5538

[9]

Method for determination of free intracellular and extracellular methylglyoxal in animal cells grown in culture [J].

Chaplen, FWR ;

Fahl, WE ;

Cameron, DC .

ANALYTICAL BIOCHEMISTRY, 1996, 238 (02) :171-178

[10]

Plasma glucose-lowering effect of tramadol in streptozotocin-induced diabetic rats [J].

Cheng, JT ;

Liu, IM ;

Chi, TC ;

Tzeng, TF ;

Lu, FH ;

Chang, CJ .

DIABETES, 2001, 50 (12) :2815-2821

← 1 2 3 4 →