Interaction of Alzheimer beta-amyloid peptide(1-40) with lipid membranes

被引:324
作者
Terzi, E
Holzemann, G
Seelig, J
机构
[1] UNIV BASEL,BIOCTR,DEPT BIOPHYS CHEM,CH-4056 BASEL,SWITZERLAND
[2] MERCK KGAA,D-64271 DARMSTADT,GERMANY
关键词
D O I
10.1021/bi971843e
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The beta-amyloid peptide beta AP(1-40), a 40-amino acid residues peptide, is one of the major components of Alzheimer's amyloid deposits, beta AP(1-40) exhibits only a limited solubility in aqueous solution and undergoes a concentration-dependent, cooperative random coil reversible arrow beta-structure transition for C-pep > 10 mu M [Terzi, E., Halzemann, G., and Seelig, J. (1995) J. Mol. Biol. 252, 633-642]. In the presence of acidic lipid, the equilibrium is shifted further toward beta-structured aggregates, We have now characterized the lipid-peptide interaction using circular dichroism (CD) spectroscopy, lipid monolayers, and deuterium and phosphorus-31 solid-state nuclear magnetic resonance (NMR). CD spectroscopy revealed a distinct interaction between beta AP(1-40) and negatively charged unilamellar vesicles, In addition to the random coil reversible arrow beta-structured aggregate equilibrium at low lipid-to-peptide (L/P) ratios, a beta-structure --> alpha-helix transition was observed at L/P > 55. beta AP(1-40) was found to insert into acidic monolayers provided the lateral pressure was low (20 mN/m), The extent of incorporation increased distinctly with the content of acidic lipid in the monolayer. However, at a lipid packing density equivalent to that of a bilayer (lateral pressure r 32 mN/m), no insertion of beta AP(1-40) was observed, The lipid molecular structure in the presence of beta AP(1-40) was studied with NMR. Phosphatidylcholine (PC) was selectively deuterated at the choline headgroup and at the cis-double bond of the oleic acyl chain and mixed with phosphatidylglycerol (PG), Phosphorus-31 NMR showed that the lipid phase retained the bilayer structure at all lipid-to-protein ratios, Deuterium MMR revealed no change in the headgroup conformation of the choline moiety or in the flexibility and ordering of the hydrocarbon chains upon the addition of beta AP(1-40). It can be concluded that beta A(1-40) binds electrostatically to the outer envelope of the polar headgroup region without penetrating between the polar groups. The data suggest a new mechanism of helix formation induced by the proper alignment of five positive charges of beta AP(1-40) on the negatively charged membrane template.
引用
收藏
页码:14845 / 14852
页数:8
相关论文
共 73 条
[1]   INTERACTION OF METAL-IONS WITH PHOSPHATIDYLCHOLINE BILAYER-MEMBRANES [J].
AKUTSU, H ;
SEELIG, J .
BIOCHEMISTRY, 1981, 20 (26) :7366-7373
[2]   GIANT MULTILEVEL CATION CHANNELS FORMED BY ALZHEIMER-DISEASE AMYLOID BETA-PROTEIN [A-BETA-P-(1-40)] IN BILAYER-MEMBRANES [J].
ARISPE, N ;
POLLARD, HB ;
ROJAS, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (22) :10573-10577
[3]   ALZHEIMER-DISEASE AMYLOID BETA-PROTEIN FORMS CALCIUM CHANNELS IN BILAYER-MEMBRANES - BLOCKADE BY TROMETHAMINE AND ALUMINUM [J].
ARISPE, N ;
ROJAS, E ;
POLLARD, HB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (02) :567-571
[4]  
BARROW CJ, 1991, SCIENCE, V253, P180
[5]   INTERACTION OF CHARGED AND UNCHARGED CALCIUM-CHANNEL ANTAGONISTS WITH PHOSPHOLIPID-MEMBRANES - BINDING EQUILIBRIUM, BINDING ENTHALPY, AND MEMBRANE LOCATION [J].
BAUERLE, HD ;
SEELIG, J .
BIOCHEMISTRY, 1991, 30 (29) :7203-7211
[6]   Binding of small basic peptides to membranes containing acidic lipids: Theoretical models and experimental results [J].
BenTal, N ;
Honig, B ;
Peitzsch, RM ;
Denisov, G ;
McLaughlin, S .
BIOPHYSICAL JOURNAL, 1996, 71 (02) :561-575
[7]   MELITTIN BINDING TO MIXED PHOSPHATIDYLGLYCEROL PHOSPHATIDYLCHOLINE MEMBRANES [J].
BESCHIASCHVILI, G ;
SEELIG, J .
BIOCHEMISTRY, 1990, 29 (01) :52-58
[8]   The serpin-enzyme complex receptor recognizes soluble, nontoxic amyloid-beta peptide but not aggregated, cytotoxic amyloid-beta peptide [J].
Boland, K ;
Behrens, M ;
Choi, D ;
Manias, K ;
Perlmutter, DH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (30) :18032-18044
[9]   MOLECULAR DETAILS OF ANESTHETIC-LIPID INTERACTION AS SEEN BY DEUTERIUM AND P-31 NUCLEAR MAGNETIC-RESONANCE [J].
BOULANGER, Y ;
SCHREIER, S ;
SMITH, ICP .
BIOCHEMISTRY, 1981, 20 (24) :6824-6830
[10]   INFLUENCE OF CHOLESTEROL ON POLAR REGION OF PHOSPHATIDYLCHOLINE AND PHOSPHATIDYLETHANOLAMINE BILAYERS [J].
BROWN, MF ;
SEELIG, J .
BIOCHEMISTRY, 1978, 17 (02) :381-384