Use of cDNA microarrays to probe and understand the toxicological consequences of altered gene expression

被引：35

作者：

Pennie, WD ^{[1
]}

机构：

[1] AstraZeneca Cent Toxicol Lab, Alderley Pk SK10 4TJ, Cheshire, England

来源：

TOXICOLOGY LETTERS | 2000年 / 112卷

关键词：

genomics; gene regulation; in vitro testing; hepatotoxicity; endocrine disruption;

D O I：

10.1016/S0378-4274(99)00199-X

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Genomic sciences offer the ability to measure quantitative modulation of transcription in cells and tissues under a wide variety of conditions. We have developed a series of custom cDNA microarrays specifically to investigate toxicity processes. Around 600 marker genes for toxicity were selected and representative cDNA clones were obtained, amplified and purified by polymerase chain reaction (PCR), before being immobilised on nylon membranes. A detailed database on biochemical function, role in disease and allelic variation has been assembled for each gene. Applications in our laboratory include mechanistic investigation of a number of toxic endpoints such as hepatotoxicity and endocrine disruption. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：473 / 477

页数：5

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