The Bacillus subtilis HBsu protein modifies the effects of α/β-type, small acid-soluble spore proteins on DNA

被引:22
作者
Ross, MA [1 ]
Setlow, P [1 ]
机构
[1] Univ Connecticut, Ctr Hlth, Dept Biochem, Farmington, CT 06032 USA
关键词
D O I
10.1128/JB.182.7.1942-1948.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
HBsu, the Bacillus subtilis homolog of the Escherichia coli HU proteins and the major chromosomal protein in vegetative cells of B, subtilis, is present at similar levels in vegetative cells and spores (similar to 5 x 10(4) monomers/genome). The level of HBsu in spores was unaffected by the presence or absence of the alpha/beta-type, small acid-soluble proteins (SASP), which are the major chromosomal proteins in spores. In developing forespores, HBsu colocalized with alpha/beta-type SASP on the nucleoid, suggesting that HBsu could modulate alpha/beta-type SASP-mediated properties of spore DNA, Indeed, in vitro studies showed that HBsu altered alpha/beta-type SASP protection of pUC19 from DNase digestion, induced negative DNA supercoiling opposing alpha/beta-type SASP-mediated positive supercoiling, and greatly ameliorated the alpha/beta-type SASP-mediated increase in DNA persistence length. However, HBsu did not significantly interfere with the alpha/beta-type SASP-mediated changes in the UV photochemistry of DNA that explain the heightened resistance of spores to UV radiation. These data strongly support a role for HBsu in modulating the effects of alpha/beta-type SASP on the properties of DNA in the developing and dormant spore.
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页码:1942 / 1948
页数:7
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