RAPD markers linked to resistance to downy mildew disease in soybean

被引:16
作者
Chowdhury, AK
Srinives, P [1 ]
Saksoong, P
Tongpamnak, P
机构
[1] Kasetsart Univ, Dept Agron, Kamphaeng Sean 73140, Nakhon Pathom, Thailand
[2] Univ Sci & Technol, Dept Genet & Plant Breeding, Patuakhali 8602, Bangladesh
[3] Kasetsart Univ, Cent Lab Greenhouse Complex, Kamphaeng Sean 73140, Nakhon Pathom, Thailand
[4] Kasetsart Univ, Dept Genet, Bangkok 10900, Thailand
关键词
bulked segregant analysis; downy mildew; Glycine max; near-isogenic lines; Peronospora manshurica; RAPD markers;
D O I
10.1023/A:1020635501050
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
To determine and utilize RAPD markers linked to resistance to downy mildew incited by Peronospora manshurica in soybean, a resistant cultivar 'AGS129' was crossed to a susceptible cultivar 'Nakhon Sawan 1' (NS1). F-2 and BC1 populations were advanced from the F-1 and evaluated for resistance to the disease. chi(2)-test demonstrated that the resistance was controlled by a single dominant gene (Rpmx). Near-isogenic lines (NILs) and bulked segregant analysis (BSA) were used to identify RAPD markers linked to the gene. Six DNA bulks namely F-5(R), F-5(S), BC6F3( R), BC6F3(S), F-2(R) and F-2(S) were set up by pooling equal amount of DNA from 8 randomly selected plants of each disease response type. A total of 180 random sequence decamer oligonucleotide primers were used for RAPD analysis. Primer OPH-02 (5' TCGGACGTGA 3') and OPP-10 (5' TCCCGCCTAC 3') generated OPH-02(1250) and OPP-10(831) fragments in donor parent and resistant bulks, but not in the recurrent parent and susceptible ones. Co-segregation analysis using 102 segregating F-2 progenies confirmed that both markers were linked to the Rpmx gene controlling downy mildew disease resistance with a genetic distance of 4.9 cm and 23.1 cm, respectively. Marker OPH-02(1250) was present in 13 of 16 resistant soybean cultivars and absent in susceptible cultivars, thus confirming a potential for MAS outside the mapping population.
引用
收藏
页码:55 / 60
页数:6
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