Promotion of regeneration and axon growth following injury in an invertebrate nervous system by the use of three-dimensional collagen gels

被引:35
作者
Blackshaw, SE [1 ]
Arkison, S [1 ]
Cameron, C [1 ]
Davies, JA [1 ]
机构
[1] UNIV SUSSEX, SUSSEX CTR NEUROSCI, BRIGHTON BN1 9QG, E SUSSEX, ENGLAND
关键词
D O I
10.1098/rspb.1997.0093
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We describe the application of three-dimensional collagen matrices to the study of nerve cord repair in the leech. Our experiments show that ganglia and connectives of the leech ventral nerve cord can be maintained for up to four weeks embedded in 3D gels constructed from mammalian type 1 collagen. Severed nerve cords embedded in the collagen gel reliably repaired within a few days of culture. The gel was penetrable by cells emigrating from the cut ends of nerves and connectives, and we consistently saw regenerative outgrowth of severed peripheral and central axons into the gel matrix. Thus, 3D gels provide an in vitro system in which we can reliably obtain repair of severed nerve cords in the dish, and visualize cell behaviour underlying regenerative growth at the damage site and which offers the possibility of manipulating the regenerating cells and their extracellular environment in various ways at stages during repair. Using this system it should be possible to test the effect on the repair process of altering expression of selected genes in identified nerve cells.
引用
收藏
页码:657 / 661
页数:5
相关论文
共 18 条
[1]  
BLACKSHAW SE, 1996, J PHYSL, V495, pP32
[2]   FINE STRUCTURE OF CENTRAL NERVOUS SYSTEM OF LEECH HIRUDO MEDICINALIS [J].
COGGESHALL, RE .
JOURNAL OF NEUROPHYSIOLOGY, 1964, 27 (02) :229-+
[3]  
DAVIES JA, 1995, NEUR ABSTR, V21, P1075
[4]   SYNAPSE FORMATION AND FUNCTION - INSIGHTS FROM IDENTIFIED LEECH NEURONS IN CULTURE [J].
FERNANDEZDEMIGUEL, F ;
DRAPEAU, P .
JOURNAL OF NEUROBIOLOGY, 1995, 27 (03) :367-379
[5]  
Guthrie S., 1994, NEUROPROTOCOLS, V4, P116
[6]   ROLE OF COLLAGENOUS MATRICES IN THE ADHESION AND GROWTH OF CELLS [J].
KLEINMAN, HK ;
KLEBE, RJ ;
MARTIN, GR .
JOURNAL OF CELL BIOLOGY, 1981, 88 (03) :473-485
[7]   CDNA LIBRARIES FROM A FEW NEURAL CELLS [J].
KORNEEV, S ;
BLACKSHAW, S ;
DAVIES, JA .
PROGRESS IN NEUROBIOLOGY, 1994, 42 (02) :339-346
[8]   cDNA libraries from identified neurons [J].
Korneev, S ;
Blackshaw, SE ;
Kaiser, K ;
Davies, JA .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1996, 263 (1366) :57-62
[9]  
LUEBKE AE, 1995, J NEUROBIOL, V25, P70
[10]   AXON SEGMENTS SPROUT AT BOTH ENDS - TRACKING GROWTH WITH FLUORESCENT D-PEPTIDES [J].
MASON, A ;
MULLER, KJ .
NATURE, 1982, 296 (5858) :655-657