A fluorescence polarization assay for cyclic nucleotide phosphodiesterases

被引：27

作者：

Huang, W ^{[1
]}

Zhang, Y ^{[1
]}

Sportsman, JR ^{[1
]}

机构：

[1] Mol Devices Corp, Sunnyvale, CA 94089 USA

来源：

JOURNAL OF BIOMOLECULAR SCREENING | 2002年 / 7卷 / 03期

关键词：

D O I：

10.1177/108705710200700305

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of the 3'-ester bond of cyclic AMP (cAMP) and cyclic GMP (cGMP), important second messengers in the transduction of a variety of extracellular signals. There is growing interest in the study of PDEs as drug targets for novel therapeutics. We describe the development of a homogeneous fluorescence polarization assay for PDEs based on the strong binding of PDE reaction products (i.e., AMP or GMP) onto modified nanoparticles through interactions with immobilized trivalent metal cations. This assay technology (IMAP) is applicable to both cAMP- and cGMP-specific PDEs, Results of the assay in 384- and 1536-well microplates are presented.

引用

页码：215 / 222

页数：8

共 17 条

[1] RECOGNITION OF PHOSPHATE GROUPS BY IMMOBILIZED ALUMINUM(III) IONS [J].