High-throughput liquid chromatography ultraviolet/mass spectrometric analysis of combinatorial libraries using an eight-channel multiplexed electrospray time-of-flight mass spectrometer

被引：28

作者：

Fang, L ^{[1
]}

Cournoyer, J ^{[1
]}

Demee, M ^{[1
]}

Zhao, J ^{[1
]}

Tokushige, D ^{[1
]}

Yan, B ^{[1
]}

机构：

[1] Discovery Partners Int, ChemRx Div, San Francisco, CA 94080 USA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2002年 / 16卷 / 15期

关键词：

D O I：

10.1002/rcm.731

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a high-throughput liquid chromatography/mass spectrometry (LC/MS) protocol for analyzing large combinatorial libraries using an eight-channel parallel LC/UV/MS (MUX-LCT) system. System configuration, linear response range in UV absorbance, LC column selection, and flow rate were optimized for 24 h/7 day unattended operations. Combinatorial libraries were analyzed on this system at a rate of 3200 compounds per day for a 3.5 min cycle time per injection. This parallel system is compared with a single-channel system in terms of performance and operation. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：1440 / 1447

页数：8

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