Quantification of the expression level of integrin receptor αVβ3 in cell lines and MR imaging with antibody-coated iron oxide particles

被引:45
作者
Benedetto, Sabrina
Pulito, Roberta
Crich, Simonetta Geninatti
Tarone, Guido
Aime, Silvio
Silengo, Lorenzo
Hamm, Jorg
机构
[1] Univ Turin, Dept Genet Biol & Biochem, I-10126 Turin, Italy
[2] Univ Turin, Ctr Med Expt, CERMS, Turin, Italy
[3] Univ Turin, Ctr Mol Imaging, Dept Chem, IFM, Turin, Italy
关键词
integrin receptors; iron oxide particles; MRI; receptor count; fluorescence activated cell sorter (FACS);
D O I
10.1002/mrm.21023
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Targeted imaging requires site-specific accumulation of a contrast agent (CA), and the properties of that agent must be selected according to the abundance of the target to obtain a signal above the detection limit of the instrument. However, numerical estimates of receptors per cell are rarely found in the literature. Integrin receptors would be particularly promising targets because of their accessibility from the blood stream and expression on activated neovascular endothelial cells. We systematically estimated the number of integrin receptors of cell lines and primary cells by flow cytometry analysis. Since integrin receptors are heterodimeric molecules, and alpha(v), forms complexes with various beta subunits, the numbers of alpha(v) and beta(3) subunits are therefore dissimilar. The observed values are 3 (.) 10(3)-1.4 (.) 10(4)/cell for alpha(v), and 5.3 (.) 10(2)-1.1 (.) 10(4) /cell for beta(3). Despite the low number of exposed receptors, we show that up to single-cell MR visualization can be achieved with the use of iron oxide beads complexed with antibodies as CAs.
引用
收藏
页码:711 / 716
页数:6
相关论文
共 20 条
[1]  
Anderson SA, 2000, MAGNET RESON MED, V44, P433, DOI 10.1002/1522-2594(200009)44:3<433::AID-MRM14>3.0.CO
[2]  
2-9
[3]  
Artemov D, 2003, CANCER RES, V63, P2723
[4]   MR molecular imaging of the Her-2/neu receptor in breast cancer cells using targeted iron oxide nanoparticles [J].
Artemov, D ;
Mori, N ;
Okollie, B ;
Bhujwalla, ZM .
MAGNETIC RESONANCE IN MEDICINE, 2003, 49 (03) :403-408
[5]   Molecular magnetic resonance imaging with targeted contrast agents [J].
Artemov, D .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2003, 90 (03) :518-524
[6]   Vascular differences detected by MRI for metastatic versus nonmetastatic breast and prostate cancer xenografts [J].
Bhujwalla, ZM ;
Artemov, D ;
Natarajan, K ;
Ackerstaff, E ;
Solaiyappan, M .
NEOPLASIA, 2001, 3 (02) :143-153
[7]   REQUIREMENT OF VASCULAR INTEGRIN ALPHA(V)BETA(3) FOR ANGIOGENESIS [J].
BROOKS, PC ;
CLARK, RAF ;
CHERESH, DA .
SCIENCE, 1994, 264 (5158) :569-571
[8]   Iron oxide MR contrast agents for molecular and cellular imaging [J].
Bulte, JWM ;
Kraitchman, DL .
NMR IN BIOMEDICINE, 2004, 17 (07) :484-499
[9]   p130Cas interacts with estrogen receptor α and modulates non-genomic estrogen signaling in breast cancer cells [J].
Cabodi, S ;
Moro, L ;
Baj, G ;
Smeriglio, M ;
Di Stefano, P ;
Gippone, S ;
Surico, N ;
Silengo, L ;
Turco, E ;
Tarone, G ;
Defilippi, P .
JOURNAL OF CELL SCIENCE, 2004, 117 (08) :1603-1611
[10]   DIFFERENTIAL DISTRIBUTION AND MODULATION OF EXPRESSION OF ALPHA1/BETA1 INTEGRIN ON HUMAN ENDOTHELIAL-CELLS [J].
DEFILIPPI, P ;
VANHINSBERGH, V ;
BERTOLOTTO, A ;
ROSSINO, P ;
SILENGO, L ;
TARONE, G .
JOURNAL OF CELL BIOLOGY, 1991, 114 (04) :855-863