Simultaneous selected reaction monitoring, MS/MS and MS3 quantitation for the analysis of pharmaceutical compounds in human plasma using chip-based infusion

被引：39

作者：

Leuthold, LA

Grivet, C

Allen, M

Baumert, M

Hopfgartner, G

机构：

[1] Univ Geneva, Sch Pharm, Lab Pharmaceut Analyt Chem Life Sci Mass Spectrom, CH-1211 Geneva 4, Switzerland

[2] Advion BioSci, Norwich NR9 3DB, Norfolk, England

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2004年 / 18卷 / 17期

关键词：

D O I：

10.1002/rcm.1587

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay method with mass spectrometric detection was developed for the quantitative analysis of a pharmaceutical compound and its major metabolite in human plasma using chip-based infusion. Liquid-liquid extraction sample preparation was found to be essential to minimize matrix suppression and to achieve a limit of quantitation (LOQ) of 2.5 ng/mL using a 100 muL plasma aliquot. The potential for simultaneous quantitation in selected reaction monitoring (SRM), tandem mass spectrometry (MS/MS) (enhanced product ion), and MS3 was investigated and found to be very beneficial in improving assay selectivity. A novel concept for monitoring quantitative assay performance using a SRM/MS3 ratio is proposed. Copyright (C) 2004 John Wiley Sons, Ltd.

引用

页码：1995 / 2000

页数：6

共 12 条

[1]

Chen S, 2001, RAPID COMMUN MASS SP, V15, P159, DOI 10.1002/1097-0231(20010130)15:2<159::AID-RCM210>3.0.CO

[2]

2-W

[3] Demonstration of direct bioanalysis of drugs in plasma using nanoelectrospray infusion from a silicon chip coupled with tandem mass spectrometry [J].