Methods for quantifying phagocytosis and bacterial killing by human neutrophils

被引：60

作者：

Hampton, MB ^{[1
]}

Winterbourn, CC ^{[1
]}

机构：

[1] Christchurch Sch Med, Free Rad Res Grp, Dept Pathol, Christchurch, New Zealand

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1999年 / 232卷 / 1-2期

关键词：

neutrophil; bacteria; phagocytosis; killing; method;

D O I：

10.1016/S0022-1759(99)00147-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

This paper reviews a variety of methods available for quantifying phagocytosis and bacterial killing by neutrophils. We outline the advantages and disadvantages of each technique, with the selection of a technique for research or analytical purposes being dependent on the information required and the resources available. A detailed protocol is provided for a comprehensive microbiological technique that measures both phagocytosis and killing in a single assay, along with a kinetic analysis for measuring and calculating separate rate constants for the two events. The kinetic analysis can be easily adapted to other methods to give the same quantitative information. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：15 / 22

页数：8

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