A technique for microsecond heating and cooling of a thin (submicron) biological sample

被引：4

作者：

Steel, BC ^{[1
]}

Bilek, MM

McKenzie, DR

dos Remedios, CG

机构：

[1] Univ Sydney, Sch Phys, Sydney, NSW 2006, Australia

[2] Univ Sydney, Inst Biomed Res, Muscle Res Unit, Sydney, NSW 2006, Australia

来源：

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 2002年 / 31卷 / 05期

关键词：

laser temperature jump; heat diffusion equation; microwave radiation; temperature pulse;

D O I：

10.1007/s00249-002-0228-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Temperature excursions of short duration are useful in exploring the effects of stress on biological systems. Stress will affect the conformation of biological molecules such as proteins, which will lead to an effect on their function. The feasibility of generating such temperature excursions is demonstrated by solving the heat diffusion equation for an aqueous layer on a silicon wafer. The silicon is rapidly heated by a laser pulse and also acts as a heat sink to quench the temperature rise. An oxide layer was used to limit the maximum temperature attained by the sample. We show that exposures above a 50 degreesC benchmark can be confined to times less than 5 mus.

引用

页码：378 / 382

页数：5

共 21 条

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