Proline-induced inhibition of glutamate release in hippocampal area CA1

被引:36
作者
Cohen, SM
Nadler, JV
机构
[1] DUKE UNIV, MED CTR, DEPT PHARMACOL, DURHAM, NC 27710 USA
[2] DUKE UNIV, MED CTR, DEPT NEUROBIOL, DURHAM, NC 27710 USA
关键词
proline; synaptic plasticity; hippocampus; glutamate; aspartate; transmitter release; paired pulse facilitation; hyperprolinemia;
D O I
10.1016/S0006-8993(97)00721-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Concentrations of proline typical of human CSF have been shown to potentiate transmission at Schaffer collateral-commissural synapses on CA1 pyramidal cells of the rat hippocampus. This study tested the hypothesis that proline enhances excitatory synaptic transmission by increasing glutamate release. Two concentrations of proline were used: a concentration typical of normal human CSF (3 mu M) and a concentration typical of CSF in persons with the genetic disorder hyperprolinemia type II (30 mu M). Continuous exposure of hippocampal slices to either concentration of proline potentiated Schaffer collateral-commissural synaptic transmission. Proline shifted the plot of field EPSP slope against fiber volley amplitude upward. Contrary to the original hypothesis, neither concentration of proline reduced paired-pulse facilitation; 30 mu M proline enhanced paired-pulse facilitation, whereas 3 mu M proline had no effect. In line with its enhancement of paired-pulse facilitation, 30 mu M proline reduced both the K+-evoked release of glutamate and aspartate from CA1 slices and the release of glutamate and aspartate from CA1 synaptosomes evoked by 4-aminopyridine. These results suggest that the proline-induced potentiation of Schaffer collateral-commissural synaptic transmission probably involves a postsynaptic, rather than a presynaptic, mechanism. Concentrations of proline normally found in human CSF little affect glutamate release. However, proline-induced inhibition of glutamate release may contribute to the neuropsychiatric disorders associated with hyperprolinemia type II. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:333 / 339
页数:7
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