Retrograde Axon Transport of Herpes Simplex Virus and Pseudorabies Virus: a Live-Cell Comparative Analysis

被引：149

作者：

Antinone, Sarah Elizabeth ^{[1
]}

Smith, Gregory Allan ^{[1
]}

机构：

[1] Northwestern Univ, Feinberg Sch Med, Dept Microbiol Immunol, Chicago, IL 60611 USA

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 03期

关键词：

BACTERIAL ARTIFICIAL CHROMOSOME; LENGTH INFECTIOUS CLONE; TEGUMENT PROTEINS; CAPSID TRANSPORT; MONOMERIC RED; TYPE-1; CONSTRUCTION; NUCLEUS; NEURONS; DNA;

D O I：

10.1128/JVI.02029-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Upon entry, neuroinvasive herpesviruses traffic from axon terminals to the nuclei of neurons resident in peripheral ganglia, where the viral DNA is deposited. A detailed analysis of herpes simplex virus type 1 (HSV-1) transport dynamics in axons following entry is currently lacking. Here, time lapse fluorescence microscopy was used to compare the postentry viral transport of two neurotropic herpesviruses: HSV-1 and pseudorabies virus (PRV). HSV-1 capsid transport dynamics were indistinguishable from those of PRV and did not differ in neurons of human, mouse, or avian origin. Simultaneous tracking of capsids and tegument proteins demonstrated that the composition of actively transporting HSV-1 is remarkably similar to that of PRV. This quantitative assessment of HSV-1 axon transport following entry demonstrates that HSV-1 and PRV share a conserved mechanism for postentry retrograde transport in axons and provides the foundation for further studies of the retrograde transport process.

引用

页码：1504 / 1512

页数：9

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