Follistatin promotes adipocyte differentiation, browning, and energy metabolism

被引：97

作者：

Braga, Melissa ^{[1
]}

Reddy, Srinivasa T. ^{[3
,4
,5
]}

Vergnes, Laurent ^{[6
]}

Pervin, Shehla ^{[1
,3
]}

Grijalva, Victor ^{[4
]}

Stout, David ^{[5
]}

David, John ^{[5
]}

Li, Xinmin ^{[6
]}

Tomasian, Venina ^{[6
]}

Reid, Christopher B. ^{[2
]}

Norris, Keith C. ^{[4
]}

Devaskar, Sherin U. ^{[6
,7
,8
]}

Reue, Karen

Singh, Rajan ^{[1
,3
]}

机构：

[1] Charles R Drew Univ Med & Sci, Div Endocrinol & Metab, Los Angeles, CA 90059 USA

[2] Charles R Drew Univ Med & Sci, Inst Life Sci, Los Angeles, CA 90059 USA

[3] Univ Calif Los Angeles, David Geffen Sch Med, Dept Obstet & Gynecol, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Los Angeles, CA 90095 USA

[5] Univ Calif Los Angeles, David Geffen Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA

[6] Univ Calif Los Angeles, David Geffen Sch Med, Dept Human Genet, Los Angeles, CA 90095 USA

[7] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA

[8] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pediat, Los Angeles, CA 90095 USA

来源：

JOURNAL OF LIPID RESEARCH | 2014年 / 55卷 / 03期

基金：

美国国家卫生研究院;

关键词：

mouse embryonic fibroblast; myostatin; brown fat; energy expenditure; uncoupling protein 1; mitochondria; WHITE ADIPOSE-TISSUE; SKELETAL-MUSCLE; MYOGENIC DIFFERENTIATION; FAT; MOUSE; MYOSTATIN; OBESITY; CELL; GROWTH; PROLIFERATION;

D O I：

10.1194/jlr.M039719

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Follistatin (Fst) functions to bind and neutralize the activity of members of the transforming growth factor-beta superfamily. Fst has a well-established role in skeletal muscle, but we detected significant Fst expression levels in interscapular brown and subcutaneous white adipose tissue, and further investigated its role in adipocyte biology. Fst expression was induced during adipogenic differentiation of mouse brown preadipocytes and mouse embryonic fibroblasts (MEFs) as well as in cold-induced brown adipose tissue from mice. In differentiated MEFs from Fst KO mice, the induction of brown adipocyte proteins including uncoupling protein 1, PR domain containing 16, and PPAR gamma coactivator-1 alpha was attenuated, but could be rescued by treatment with recombinant FST. Furthermore, Fst enhanced thermogenic gene expression in differentiated mouse brown adipocytes and MEF cultures from both WT and Fst KO groups, suggesting that Fst produced by adipocytes may act in a paracrine manner. Our microarray gene expression profiling of WT and Fst KO MEFs during adipogenic differentiation identified several genes implicated in lipid and energy metabolism that were significantly downregulated in Fst KO MEFs. Furthermore, Fst treatment significantly increases cellular respiration in Fst-deficient cells.(jlr) Our results implicate a novel role of Fst in the induction of brown adipocyte character and regulation of energy metabolism.

引用

页码：375 / 384

页数：10

共 49 条

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