Effects of human neutrophil peptide-1 on the expression of interstitial collagenase and type I collagen in human dermal fibroblasts

被引：45

作者：

Oono, T ^{[1
]}

Shirafuji, Y ^{[1
]}

Huh, WK ^{[1
]}

Akiyama, H ^{[1
]}

Iwatsuki, K ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Med & Dent, Dept Dermatol, Okayama 7008558, Japan

来源：

ARCHIVES OF DERMATOLOGICAL RESEARCH | 2002年 / 294卷 / 04期

关键词：

defensin; human neutrophil peptide; collagen; collagenase; wound healing;

D O I：

10.1007/s00403-002-0310-6

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Human neutrophil peptide-1 (HNP-1), a defensin with antimicrobial properties, is also thought to promote wound healing. To elucidate the mechanism by which wound healing is facilitated by this factor, we investigated the effect of HNP-1 on the expression of interstitial collagenase (matrix metalloproteinase 1, MMP-1), collagen types I and III, and tissue inhibitor of metalloproteinase 1 (TIMP-1) by cultured fibroblasts by means of RT-PCR and ELISA. Our results showed that synthetic HNP-1 increased the expression of proalphaI(I) collagen mRNA and protein. In contrast, the expression of MMP-1 was decreased at both the mRNA and protein levels. Our observations suggest that HNP-1 may promote wound repair by enhancing extracellular matrix deposition and by controlling its degradation.

引用

页码：185 / 189

页数：5

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