Sensitive detection of protein by an aptamer-based label-free fluorescing molecular switch

被引：122

作者：

Li, Bingling

Wei, Hui

Dong, Shaojun ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2007年 / 01期

关键词：

D O I：

10.1039/b612080f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report an aptamer-based method for the sensitive detection of proteins by a label-free fluorescing molecular switch (ethidium bromide), which shows promising potential in making protein assay simple and economical.

引用

页码：73 / 75

页数：3

共 36 条

[1] Fluorescent dyes specific for quadruplex DNA [J].

Arthanari, H ;

Basu, S ;

Kawano, TL ;

Bolton, PH .

NUCLEIC ACIDS RESEARCH, 1998, 26 (16) :3724-3728

[2]

Baker BR, 2006, J AM CHEM SOC, V128, P3138, DOI 10.1021/ja056957p

[3] Ability of thrombin to act as molecular chaperone, inducing formation of quadruplex structure of thrombin-binding aptamer [J].

Baldrich, E ;

O'Sullivan, CK .

ANALYTICAL BIOCHEMISTRY, 2005, 341 (01) :194-197

[4] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].

BOCK, LC ;

GRIFFIN, LC ;

LATHAM, JA ;

VERMAAS, EH ;

TOOLE, JJ .

NATURE, 1992, 355 (6360) :564-566

[5] Photophysics of ethidium bromide complexed to ct-DNA: a maximum entropy study [J].

Byrne, CD ;

de Mello, AJ .

BIOPHYSICAL CHEMISTRY, 1998, 70 (03) :173-184

[6] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[7] Synthetic DNA aptamers to detect protein molecular variants in a high-throughput fluorescence quenching assay [J].

Fang, XH ;

Sen, A ;

Vicens, M ;

Tan, WH .

CHEMBIOCHEM, 2003, 4 (09) :829-834

[8] Study of binding stoichiometries of the human immunodeficiency virus type 1 reverse transcriptase by capillary electrophoresis and laser-induced fluorescence polarization using aptamers as probes [J].

Fu, H ;

Guthrie, JW ;

Le, XC .

ELECTROPHORESIS, 2006, 27 (02) :433-441

[9]

GAO ZH, 2005, CURR PROTEOMICS, V2, P31

[10] INTERACTION OF THE DYE ETHIDIUM-BROMIDE WITH DNA CONTAINING GUANINE REPEATS [J].

GUO, Q ;

LU, M ;

MARKY, LA ;

KALLENBACH, NR .

BIOCHEMISTRY, 1992, 31 (09) :2451-2455

← 1 2 3 4 →